适用于细粒棘球蚴囊液蛋白质组分析的双向电泳技术  

Two-dimensional electrophoresis for proteomics analysis of hydatid fluid of the Echinococcus granulosus

作　　者：李居怡[1] 刘巧媚 黄文静[3] 阮周莹 邓艾平[1] 

机构地区：[1]武汉市中心医院药学部,湖北武汉430021 [2]武汉市第三医院医务处,湖北武汉430061 [3]武汉市中心医院内分泌科,湖北武汉430021 [4]武汉市中心医院眼科,湖北武汉430021

出　　处：《西安交通大学学报（医学版）》2015年第5期711-714,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30960360);武汉市临床医学科研项目(No.WX11A02;WZ15D19)~~

摘　　要：目的建立适用于细粒棘球蚴囊液蛋白质组的双向电泳分离条件。方法冷冻干燥法制备囊液总蛋白提取物,观察不同的蛋白样品处理方法、IPG胶条pH范围和电泳上样量对双向电泳图谱的影响。结果经ReadyPrepTM2-D Cleanup Kit处理的提取物上样量为400μg,在pH7-10范围的胶条进行双向电泳分离,可获取蛋白斑点较多、重复性较好的二维电泳图谱。结论建立的细粒棘球蚴囊液双向电泳技术及图谱,可为细粒棘球蚴虫蛋白质组学的研究提供理论依据。Objective To establish two-dimensional electrophoresis（2-DE）technology for hydatid fluid of the parasite Echinococcus granulosus. Methods We extracted total proteins of Echinococcus granulosus by lyophilization and studied the changes of 2-DE picture under different methods of dealing with the protein samples,pH range of IPG strip and quantitative loading of the samples.Results The two-dimensional collection of illustrative plate with better resolution and repetition was achieved when the hydatid fluid dealt with ReadyPrepTM2-D Cleanup Kit was analyzed using 400μg of quantitative loading and IPG strips pH7-10.Conclusion The established 2-DE method and maps of hydatid fluid of the parasite Echinococcus granulosus can provide theoretical evidence for proteomic study of Echinococcus granulosus.

关 键 词：细粒棘球蚴 囊液 蛋白质组学 双向电泳 

分 类 号：R183.4[医药卫生—流行病学]