真皮多潜能细胞注射对皮肤弹性的影响  

Effect of Dermal Multipotent Cells Transplantation on Skin Elasticity in Aged Murine

作　　者：薛斯亮[1] 李利[1] 黄珊珊[1] 周成霞[1] 

机构地区：[1]四川大学华西医院皮肤科,成都610041

出　　处：《四川大学学报（医学版）》2015年第5期706-709,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.81071312)资助

摘　　要：目的探讨真皮来源多潜能细胞(dermal multipotent cells,DMCs)注射对皮肤弹性的影响。方法从3d龄BALB/c小鼠真皮中分离培养获得DMCs,取第3代DMCs观察形态和成脂、成骨分化。将36只12月龄BALB/c小鼠分为DMCs组(n=12)、成纤维细胞(FBs)组(n=12)及对照组(n=12),分别将104个DMCs、104个FBs和100μL DMEM培养基注射入小鼠双下肢之间背部中央部位的皮下作单点注射。于2周及4周使用MPA580皮肤弹性测试仪检测小鼠皮肤弹性变化。结果 DMCs接受诱导分化后,分别成脂、成骨。接受DMCs注射的小鼠皮肤弹性较对照组在2周后有较大增加(P=0.000),但与FBs组差异无统计学意义(P=0.216)。4周后皮肤弹性不仅较对照组增加(P=0.000),且亦较FBs组增加(P=0.031)。结论 DMCs注射在小鼠皮下可以增加皮肤的弹性。Objective To investigate the elasticity changes in aged dermis after injection of dermal multipotent cells （DMCs）. Methods Dermal multipotent cells were isolated and cultured from 3-day-old BALB/c mice and then transplanted into the dermis of aged （12-week-old） BALB/c mice. Adult fibroblasts （FBs） were employed as control. At 2 and 4 weeks after the transplantation, we examined dermal elasticity by MPA580 skin test machine. Results The skin elasticity were improve at 2 weeks after the transplantion in both DMCs group and FBs group （P= 0. 000） but have no statistical difference between these two groups （P = 0. 216）. The different effect between these two groups appeared in 4 weeks after the transplantation （P=0. 031）. Conclusion Dermal multipotent cells appear more effective than fibroblasts in increasing skin elasticity.

关 键 词：真皮 多潜能 分化 

分 类 号：R751[医药卫生—皮肤病学与性病学]