Anti-miR-145促进人气道平滑肌细胞增殖及骨桥蛋白合成  

Anti-miR-145 promotes human airway smooth muscle cell proliferation and osteopontin synthesis in vitro

作　　者：陈培芬[1] 邱智辉[2] 黄国华[3] 张香梅[4] 彭武建[1] 曾辉[1] 赖文岩[5] 

机构地区：[1]广东深圳市第三人民医院内二科,广东深圳518112 [2]广东深圳市第三人民医院内一科,广东深圳518112 [3]南方医科大学附属南方医院呼吸科,广东广州510515 [4]广东深圳市第三人民医院病理科,广东深圳518112 [5]南方医科大学附属南方医院心内科实验室,广东广州510515

出　　处：《南方医科大学学报》2015年第7期1073-1075,共3页Journal of Southern Medical University

基　　金：深圳市科技计划(201102064)

摘　　要：目的：观察miR-145抑制剂（Anti-miR-145）对人支气管平滑肌细胞（HASMCs）的影响，探讨其在哮喘气道重塑中的作用。方法分为对照组和实验组，实验组加入不同浓度的Anti-miR-145（10-100 nmol/L）。CCK-8法检测细胞增殖，流式细胞术检测细胞凋亡，Western blotting检测骨桥蛋白合成。结果与对照组比较，10 nmol/L和50 nmol/L Anti-miR-145显著促进HASMCs增殖及骨桥蛋白合成（P〈0.05或P〈0.01），50 nmol/L Anti-miR-145显著抑制HASMCs凋亡（P〈0.01）。结论 Anti-miR-145通过刺激HASMCs增殖和骨桥蛋白合成，抑制其凋亡，可能在哮喘气道重塑中发挥重要作用。Objective To investigate the effect of anti-miR-145 on human airway smooth muscle cell （HASMC） proliferation and osteopontin systhesis in vitro and explore the mechanisms. Methods HASMCs were treated with 10-100 nmol/L anti-miR-145, and the cell proliferation and apoptosis were investigated using a CCK-8 assay and flow cytometry, respectively. The changes in osteopontin synthesis after the treatment was quantified with Western blotting. Results Treatment with 10 and 50 nmol/L anti-miR-145 significantly promoted the proliferation and osteopontin synthesis in HASMCs （P〈0.05 or〈0.01）, and 50 nmol/L anti-miR-145 obviously inhibited the cell apoptosis （P〈0.01）. Conclusion Anti-miR-145 promotes HASMC proliferation and osteopontin synthesis and inhibits HASMC apoptosis in vitro, indicating the important role of anti-miR-145 in the pathogenesis of airway remodeling.

关 键 词：anti-miR-145 气道平滑肌细胞 增殖 骨桥蛋白 

分 类 号：R562.25[医药卫生—呼吸系统]