水貂绿脓杆菌的分离鉴定  

Isolation and Identification of Pseudomonas aeruginosa in Minks

作　　者：耿海东[1] 李景玉[2] 解林红[1] 徐钰[1] 李元果[3] 王铁成[3] 尚丽元 初冬[1] 孙贺廷[2] 

机构地区：[1]国家林业局野生动物疫源疫病监测总站,辽宁沈阳110034 [2]吉林省白城市畜牧总站,吉林白城137000 [3]国家林业局长春野生动物疫病研究中心,吉林长春130122 [4]吉林省莫莫格国家级自然保护区管理局,吉林白城1137316

出　　处：《现代畜牧兽医》2015年第8期11-15,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：国家科技支撑计划项目(项目编号:2013BAD12B04)

摘　　要：为确诊引起该养殖场水貂大量死亡的主要病因,对吉林省某貂场送检的10只水貂进行剖检,根据死亡水貂临床表现、剖检变化,经16s r RNA PCR检测、小鼠的致死性试验、细菌分离及生化试验确诊为绿脓杆菌感染。对该致病菌进行分离纯化,测定小鼠半数致死量为1×105.48cfu/m L。In order to identify the main bacterial pathogens that caused the death of minks in a farm, we dissected ten dead minks that submitted by one farm of jilin province. These isolates be- longed to Pseudomonas aeruginosa according to the examination of fresh samples by clinical analy- sis, pathological observation, and then the bacteria were identified by drug biochemical test, pathogenicity test and 16S rRNA sequencing. Pathogenicity tests indicated the isolates could cause mouse median lethal dose in healthy mice and its median lethal dose was 1×105.48cfu/mL.

关 键 词：绿脓杆菌 分离鉴定 致病性 水貂 

分 类 号：S852.617[农业科学—基础兽医学]