HPLC法测定气血双补酒中人参皂苷Rg_1、Re和Rb_1的含量  

Determination of Ginsenoside Rg_1,Re and Rb_1 in Qixue Shuangbu Tinctura by HPLC

作　　者：高杨[1] 陈林伟 陈丹妮[2,3] 王彬[2,3] 金俊杰 秦昆明[2,3,4] 蔡宝昌[2,3,4] 

机构地区：[1]南京中医药大学附属南京市中西医结合医院,江苏南京210014 [2]南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210023 [3]国家中医药管理局中药炮制标准重点研究室,江苏南京210023 [4]南京海昌中药集团有限公司江苏省企业研究生工作站,江苏南京210061

出　　处：《中华中医药学刊》2015年第9期2095-2097,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家海洋局公益性行业专项(201305007-3);江苏省科技支撑计划-工业项目(BE2012011);南京市科技公共服务平台项目(201105007);南京市科技计划项目(201304045)

摘　　要：目的：建立气血双补酒中人参皂苷Rg1、Re和Rb1的含量测定方法,更有效地控制该制剂的质量。方法：采用Merck C18柱,以乙腈-水为流动相进行梯度洗脱,检测波长为203 nm,柱温35℃。结果：人参皂苷Rg1在1.290～6.450μg之间、人参皂苷Re在0.492 0～2.952 0μg之间、人参皂苷Rb1在1.485 0～7.425 0μg之间呈良好的线性关系;样品精密度试验、稳定性试验、重复性试验中RSD均小于2%;人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的平均回收率分别为98.05%、97.84%和99.35%,且RSD均小于2%（n=6）。结论：采用HPLC同时测定气血双补酒中人参皂苷Rg1、Re和Rb1的含量,方法简便,重现性好,可用于气血双补酒的生产中质量控制及质量评价。Objective： To establish an HPLC method for the determination of ginsenoside Rg1,Re and Rb1 in Qixue Shuangbu Tinctura to more effectively control the quality of this preparation. Methods： HPLC was used to do the quantitative analysis. The Merck C18 column was used and mobile phase was composed of acetonitrile-water; detection wavelength was at 203 nm at 35 ℃. Results： The linear range of ginsenoside Rb1 was 1. 290 ～ 6. 450 μg; the linear range of ginsenoside Re was 0. 492 0 ～ 2. 952 0 μg; the linear range of ginsenoside Rg1 was 1. 485 0 ～ 7. 425 0 μg. The sample precision test,stability test and repeatability test of RSD were less than 2%. The average recoveries of ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 were 98. 05%,97. 84%,99. 35% and 99. 35% respectively and the RSD was less than 2%（n = 6）. Conclusion： Ginsenoside Rg1,Re and Rb1 are determined simultaneously in Qixue Shuangbu Tinctura by HPLC. The method and reproducibility is available. This method can be used for quality control and evaluation of Qixue Shuangbu Tinctura.

关 键 词：气血双补酒 HPLC 人参皂苷RG1 人参皂苷RE 人参皂苷RB1 

分 类 号：R284.1[医药卫生—中药学]