蜜炙紫菀水煎剂对大肠癌LOVO细胞凋亡及迁移的影响  

Effects of Honey-fried Radix Asteris Decoction(HFRAD) on Cell Apoptosis and Migration in Colorectal Cancer LOVO cells

作　　者：钱树树 彭林[1] 杨映映 秦甜甜 刘莉嘉 白松绵 王如峰[1] 蒋燕[1] 胡秀华[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中华中医药学刊》2015年第9期2146-2150,I0009,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81274044);国家级大学生创新创业训练计划项目(20131002602)

摘　　要：目的：探讨蜜炙紫菀水煎剂对大肠癌LOVO细胞凋亡及迁移的影响方法：应用荧光显微镜观察DAPI染色后蜜炙紫菀水煎剂作用前后对LOVO细胞凋亡情况的影响;流式细胞仪检测蜜炙紫菀水煎剂对LOVO细胞凋亡率的影响;划痕实验和Transwell小室法检测不同质量浓度的蜜炙紫菀水煎剂处理LOVO细胞后对大肠癌LOVO细胞的迁移情况的影响。结果：荧光显微镜下观察发现蜜炙紫菀水煎剂实验组和空白对照组均未见细胞核凝集、染色体碎裂等细胞凋亡改变;流式细胞术结果也发现,不同浓度蜜炙紫菀水煎剂处理LOVO细胞48 h后,在细胞周期图G0期均未出现二倍峰,且凋亡率未见明显改变;浓度为5～30 mg·m L-1的蜜炙紫菀水煎剂处理LOVO细胞24、48 h后,与对照组相比,划痕实验结果显示大肠癌LOVO细胞的划痕距离较大,划痕未见明显愈合,均可以抑制大肠癌LOVO细胞的迁移,在蜜炙紫菀水煎剂浓度为40 mg·m L-1和80 mg·m L-1时,会导致大肠癌LOVO细胞非凋亡性死亡,Transwell小室结果显示,药物实验组穿透Transwell小室上层膜到达下室的LOVO细胞数明显少于空白对照组,蜜炙紫菀水煎剂浓度为30 mg·m L-1的实验组迁移率最低,为50.7%。结论：不同浓度的蜜炙紫菀水煎剂对大肠癌LOVO细胞的凋亡没有影响,但在高浓度时可以导致非凋亡性细胞死亡。同时,确定一定浓度的蜜炙紫菀水煎剂可以有效抑制大肠癌LOVO细胞的迁移,为有效预防大肠癌复发提供实验基础。Objective： To investigate the effect of different concentrations of honey-fried Radix asterised decoction（HFRAD） on cell apoptosis and migration in colorectal cancer LOVO cells in vitro. Method： DAPI stain method and flow cytometry were applied to observe apoptotic body and apoptotic ratio of LOVO cell. Cell migration was evaluated using scratch test and transwell test in LOVO cells. Result： Compared with the control group,DAPI staining of LOVO cells treated with 0. 2,0. 4,0. 8,1. 6,3. 2,6. 4,12. 8 mg·m L-1HFRAD for 48 h indicated that HFRAD treatment did not induce nucleus condensation and chromosome fragmentation under the fluorescence microscope. Flow cytometry assay showed that the hypodiploid peak had been not observed in the G0 cell cycle diagram of the LOVO cells treated by 20 mg·m L-1and 40 mg·m L-1HFRAD for 48 h. Moreover,we found HFRAD had no effect on the apoptotic ratio. The cell scratch analysis indicated LOVO cells treated by 5 mg·m L-1-30 mg·m L-1HFRAD for 24 h and 48 h had lower wound healing rate and could inhibit cell migration. LOVO cells treated with 40 mg·m L-1and 80 mg·m L-1HFRAD for 24 h and 48 h were found non-apoptotic cell death. Transwell assay indicated that migration rate of HFRAD treated-LOVO cells was obviously reduced And the lowest migration 50. 7% was identified in 30 mg·m L-1HFRAD treated-LOVO cells.Conclusion： Different concentrations of HFRAD had no effect on cell apoptosis in vitro,but high concentration of HFRAD would cause non-apoptotic cell death in LOVO cells. Interestingly the certain concentration of HFRAD could effectively affect cell migration of LOVO cells.

关 键 词：大肠癌LOVO细胞 蜜炙紫菀水煎剂 细胞凋亡 细胞迁移 

分 类 号：R285.5[医药卫生—中药学]