益肝降脂方预处理对酒精性脂肪肝SD大鼠HSP70表达的影响  

Effects of Preconditioning with Yigan Jiangzhi Formula on Hot Shock Protein 70 in alcoholic fatty liver of Rats

作　　者：尹纪红[1] 赵远红[2] 李正[2] 李全征[1] 侯珊珊[1] 张小瑞[1] 杨彩霞[1] 

机构地区：[1]天津中医药大学,天津300193 [2]天津中医药大学第一附属医院,天津300193

出　　处：《中华中医药学刊》2015年第9期2231-2234,I0022,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：天津市应用基础及前沿技术重点项目(11JCZDJC19900)

摘　　要：目的:观察分析益肝降脂方(YGJZF)预处理对酒精性脂肪肝(AFLD)SD大鼠热休克蛋白70(HSP70)mRNA和蛋白表达的影响,探讨YGJZF对内源性保护机制中效应物质HSP70调控的意义。方法:SPF级雄性SD大鼠120只适应性喂养1周后,随机分为空白对照组4只、模型组22只、中药YGJZF干预组94只(包括第3周干预组35只,第6周干预组31只,第9周干预组28只)。模型组给予酒精灌胃+高脂饮食造模,中药组在造模进行同时于实验第3周、第6周、第9周起给予干预,于第6周末、9周末、12周末随机取材观察大鼠一般情况;分别采用RT-PCR法和Western blot法检测各组大鼠肝组织HSP70 mRNA和蛋白的表达情况;HE染色观察大鼠肝脏组织病理形态改变。结果:模型组及中药YGJZF不同时段干预组大鼠肝组织HSP70 mRNA和蛋白表达水平均明显高于空白对照组,差异有统计学意义(P<0.05);与模型组比较,YGJZF不同时段用药组肝组织HSP70 mRNA和蛋白表达水平均降低,且各中药用药组间比较有统计学意义(P<0.05),中药第3周干预组降低更加明显;HE染色显示模型组肝脏损伤最严重,中药第3周干预组肝细胞变性、坏死程度明显减轻,肝组织病变明显改善。结论:YGJZF预处理可降低和推迟慢性酒精刺激引起的HSP70的表达,减轻了酒精性肝损伤,实现了药物预适应样保护作用,早期干预显示效果明显;内源性信号传导路径中效应物质HSP70对慢性酒精刺激引起的酒精性肝损伤具有一定的缓解作用,这可能是YGJZF预处理减轻酒精性肝损伤的重要原因。Objective： To observe and analyze the effects of Yigan Jiangzhi Formula（YGJZF） precondition in heat shock protein 70（HSP70） mRNA and protein of alcoholic fatty liver disease（AFLD） SD rats and to explore the significance of the effect of endogenous protective mechanism in the regulation of HSP70 substance. Methods： 120 SPF male SD rats were fed one week after adaptation and were randomly divided into control group 4,model group 22,YGJZF group 94（including intervention group 3 weeks 35,intervention group 6 weeks 31,intervention group 9 weeks 28）. Model group were given alcohol ＋ high-fat diet fed and the YGJZF group was given intervention at 3rd,6th and 9th week when modeling. In the experiment,at 6th,9th and 12 th weekend,we randomly killed rats,using Western blot technique to detect the expression of protein blotting HSP70 in liver tissue of rats and HE stained of rat liver histopathology. Results： Compared with the blank control group,in the first 3 weeks（3-12 weeks） intervention,hepatic HSP70 mRNA and protein expression of rats were increased significantly and the difference was statistically significant（P〈0. 05）. Compared with the model group,YGJZF treatment group＇s HSP70 mRNA and protein expression of liver at different time decreased and there was significant difference（P〈0. 05）. The decrease in YGJZF treatment group was obvious at 3rd week. Meantime,compared with the other groups,model group＇s liver tissue had the most serious pathological damage. Conclusion： YGJZF preconditioning can reduce the effects of endogenous signaling pathways substances HSP70 protein and mRNA expression and reduce alcohol liver injury,the protecting the pharmacological preconditioning and achieving the obvious early intervention effect. HSP70 may be an important reason of YGJZF pretreatment for reducing alcohol-induced liver injury.

关 键 词：益肝降脂方 酒精性脂肪肝 热休克蛋白70 内源性保护 预处理 

分 类 号：R285.5[医药卫生—中药学]