Cloning,Sequencing and Analysis of DDX3Y Gene in Five Male Equus Animals  

作　　者：Huamin GUO Yanglin CHEN Shuyu LI Hongmei HAN Jindun ZHANG Lihong FAN Lixia ZHAO Xihe LI 

机构地区：[1]Research Center for Animal Genetic Resources of Mongolia Plateau,Inner Mongolia University [2]Inner Mongolia Saikexing Reproductive Biotechnology Co,Ltd.

出　　处：《Agricultural Biotechnology》2015年第4期64-69,共6页农业生物技术（英文版）

基　　金：Supported by Project of Science and Technology Department of Inner Mongolia Autonomous Region

摘　　要：[ Objective] This study analyzed the differences in DDX3 Y gene sequences among male horse, male donkey, male mule, male hinny and male foal to investigate the relationship between DDX3Y gene structure and the mechanism of infertility in male Equus fetus × asirms. [ Method] Partial sequences of DDX3Y gene in male horse, male donkey, male mule, male hinny and male foal were obtained by genome comparison, sequencing and cloning, and compared by bioinformatics methods. [ Result] The CDS regions varied in 5 base pairs and two amino acids. DDX3Y protein was predicted to be an unstable protein. In addition, the results also showed that DDX3 Y gene in male horse displayed a closer phylogenetic relationship to male mule and male foal; DDX3Y gene in male donkey displayed a closer phylogenetic relationship to male hinny. [ Conclusion] This study provided scientific basis for further exploring the function of DDX3 Y gene and the mecha- nism of reproductive regulation in male Equus ferus × asinus.[ Objective] This study analyzed the differences in DDX3 Y gene sequences among male horse, male donkey, male mule, male hinny and male foal to investigate the relationship between DDX3Y gene structure and the mechanism of infertility in male Equus fetus × asirms. [ Method] Partial sequences of DDX3Y gene in male horse, male donkey, male mule, male hinny and male foal were obtained by genome comparison, sequencing and cloning, and compared by bioinformatics methods. [ Result] The CDS regions varied in 5 base pairs and two amino acids. DDX3Y protein was predicted to be an unstable protein. In addition, the results also showed that DDX3 Y gene in male horse displayed a closer phylogenetic relationship to male mule and male foal; DDX3Y gene in male donkey displayed a closer phylogenetic relationship to male hinny. [ Conclusion] This study provided scientific basis for further exploring the function of DDX3 Y gene and the mecha- nism of reproductive regulation in male Equus ferus × asinus.

关 键 词：Male mule Male hinny Male foal DDX3Y INFERTILITY 

分 类 号：Q78[生物学—分子生物学] S852.651[农业科学—基础兽医学]