造血系统特异性miR-155通过靶向CUX1参与小鼠巨噬细胞终末分化  

Hematopoietic System Specific mi R-155 Regulated Macrophage Cells Terminal Differentiation by Targeting CUX1

作　　者：何洁华[1] 廖建友[1] 吴珏[1] 邓伟溪[1] 

机构地区：[1]中山大学孙逸仙纪念医院医学研究中心,510120

出　　处：《中国卫生标准管理》2015年第23期189-190,共2页China Health Standard Management

基　　金：国家自然科学基金项目(C060604);广东省自然科学基金项目(2014A030313175)

摘　　要：目的筛选靶向CUX1的造血系统特异性mi RNAs,探讨其调控巨噬细胞终末分化的分子机制。方法双荧光报告检测筛选靶向CUX1的造血作用相关mi RNAs,Real-time PCR检测巨噬细胞分化过程中mi R-155和CUX1 m RNA表达,免疫印迹检测CUX1蛋白表达水平。结果双荧光报告检测筛选到5个脾脏和胸腺特异或富集mi RNAs在CUX1 3’UTR上有作用位点,进一步在PMA诱导HL-60分化为巨噬细胞样细胞模型中发现,mi R-155随着巨噬细胞分化表达水平逐渐上调,与之相反CUX1蛋白和m RNA表达水平逐渐下调,且mi R-155能直接调控CUX1蛋白水平。结论造血系统特异mi R-155通过转录后调控CUX1蛋白表达参与巨噬细胞终末分化。Objective To explore the molecular mechanism of hematopoietic system specific miRNAs which regulate macrophage cel development by targeting CUX1.Methods Screening for hematopoietic specific miRNAs targeting CUX1 with Dual-Luciferase Reporter Assay, expression of miR-155 、CD11b、CD14 and CUX1 mRNA were tested by Real-time PCR, expression of CUX1 protein was detected by Western Blot.ResultsWe figured out five spleen and thymus specific or enrich miRNAs can target 3’UTR of CUX1 by Dual-Luciferase Reporter Assay. And in the macrophage differentiation model, we found that expression of miR-155 increased gradualy, while CUX1 decreased during differentiation. Further western blot assay showed that CUX1 protein were regulated by miR-155 directly.ConclutionHematopoietic system specific miR-155 regulates macrophage terminal differentiation by repressing CUX1 at post-transcriptional level.

关 键 词：造血系统特异miR-155 CUX1 巨噬细胞 终末分化 

分 类 号：R446[医药卫生—诊断学]