人巨细胞病毒检测在婴幼儿肺炎中的应用  

Detection of Human Cytomegalovirus in Infants with Pneumonia

作　　者：韩敏 

机构地区：[1]呼伦贝尔市人民医院,内蒙古呼伦贝尔021008

出　　处：《广东微量元素科学》2015年第8期34-36,共3页Trace Elements Science

摘　　要：目的探讨HCMV检测在婴幼儿肺炎中的诊断价值和临床应用。方法对2013年1月—12月180例在呼伦贝尔市人民医院诊断为婴幼儿肺炎的患儿进行外周血HCMV-IgM和尿液HCMV-DNA的检测,分别采用酶联免疫捕获法(ELISA)、荧光定量聚合酶链反应(PCR)对收集的血清及尿液进行检测。结果 180例婴幼儿肺炎中确诊为HCMV感染的72例,其中54例血清HCMV-IgM检测阳性,阳性率占75%,62例尿液HCMV-DNA检测阳性,阳性率占86%。对检测出HCMV的患儿进行临床跟踪调查发现用更昔洛韦等药物治疗后疗效显著。结论婴幼儿肺炎中HCMV感染所占比率高,通过血清HCMV-IgM及尿液HCMV-DNA的联合检测能够给临床提供一个治疗依据,有助于早期诊断、早期治疗,对减少并发症及预后至关重要。Objective To explore the diagnostic value of detection of HCMV in infant pneumonia and clinical application. Methods In 2013 January- December 180 cases in our hospital diagnosis for children with infantile pneumonia and serum HCMV Ig M and urine hcmv- dna detection,respectively by enzyme linked immuno capture assay（ ELISA） and fluorescence quantitative polymerase chain reaction（ PCR） to collect serum and urine were detected. Results 180 cases of pneumonia in infants diagnosed as HCMV infection in 72 cases,of which 54 cases of serum HCMV- Ig M positive,positive rate was75%,62 cases of urine HCMV- DNA positive,positive rate accounted for 86%. To detect HCMV with clinical follow- up survey found significantly after treatment with ganciclovir and other drugs.Conclusion HCMV infection in infants with pneumonia accounted for high rate,it can provide a basis for treatment to clinical by combined detection of serum HCMV Ig M and urine hcmv- dna,helpful to early diagnosis and early treatment to reduce complications and prognosis in patients with essential.

关 键 词：婴幼儿肺炎 HCMV-IGM HCMV-DNA检测 

分 类 号：R375.2[医药卫生—病原生物学]