几种杀虫剂胁迫对黄瓜幼苗抗氧化酶活性和相关生理指标的影响  

Effects of pesticides on antioxidant enzyme activities and related physiological indices on cucumber seedlings

作　　者：王宏民[1] 李亚芳[2] 张仙红[2] 李赫[2] 赵建华[2] 

机构地区：[1]山西农业大学经济管理学院,太谷030801 [2]山西农业大学农学院,太谷030801

出　　处：《中国生态农业学报》2015年第9期1185-1190,共6页Chinese Journal of Eco-Agriculture

基　　金：山西省自然基金项目(2013011034-3);山西省科技攻关项目(20150311010-6);山西省农业科技成果转化项目(No.714197)资助

摘　　要：明确杀虫剂多次施用后对黄瓜幼苗抗氧化酶活性和相关生理指标的影响,对指导黄瓜（Cucumis sativus L.）合理用药具有重要作用。本研究以室内盆栽的黄瓜幼苗为试材,研究了几种常用化学杀虫剂吡虫啉、啶虫脒、溴氰菊酯及生物杀虫剂玫烟色棒束孢对黄瓜幼苗抗氧化酶活性（SOD、POD、CAT）及丙二醛MDA、脯氨酸和蛋白质含量的影响。结果表明：不同杀虫剂对黄瓜幼苗抗氧化酶活性及相关生理指标的影响不同。当供试3种化学杀虫剂胁迫黄瓜幼苗2 d时,其体内SOD、POD、CAT活性与对照无显著差异;当胁迫时间延长到4 d时,溴氰菊酯和啶虫脒处理的黄瓜幼苗体内3种抗氧化酶活均明显升高且与对照差异显著;当胁迫时间延长到6 d时,3种化学杀虫剂均造成黄瓜幼苗体内SOD、POD活性升高且与对照差异显著,其中溴氰菊酯影响较吡虫啉和啶虫咪严重;胁迫8-10 d后,各处理黄瓜幼苗体内3种抗氧化酶活性均开始下降或恢复正常。同时,3种化学杀虫剂胁迫黄瓜幼苗4-8 d时,MDA和脯氨酸含量升高并与对照差异显著;胁迫10 d时,啶虫咪和溴氰菊酯胁迫的黄瓜幼苗MDA含量仍显著高于对照,而脯氨酸含量开始下降并趋于正常。此外,3种化学杀虫剂胁迫黄瓜幼苗2-8 d时均造成其蛋白质含量的降低,而生物杀虫剂玫烟色棒束孢胁迫对黄瓜幼苗SOD、POD、CAT活性及MDA、脯氨酸和蛋白质含量均没有显著影响。可见生物杀虫剂玫烟色棒束孢较3种化学杀虫剂对黄瓜幼苗安全,黄瓜幼苗受化学杀虫剂胁迫后需要一个过程才能修复杀虫剂对其自身造成的伤害。Understanding antioxidant enzymes activities and other related physiological indexes of cucumber（Cucumis sativus L.） seedling under repeatedly application of insecticides is critical for safe application of pesticides. In order to explore the effects of three chemical pesticides（Deltamethrin, Acetamiprid and Imidacloprid） and one biological pesticide（Isaria fumosorosea） on cucumber seedlings, activities of antioxidant enzymes（SOD, POD and CAT） and contents of malondiadehyde（MDA）, proline（Pro） and protein in pesticides stressed cucumber seedlings were investigated under laboratory conditions. The results showed that different insecticides had different effects on antioxidant enzymes activities and related physiological indexes of cucumber seedlings. For cucumber seedlings under stresses of three chemical insecticides for two days, the activities of three antioxidant enzymes were not significantly different from those of the control. When stress was prolonged to four days, the activities of three antioxidant enzymes in cucumber seedlings under Decamethrin and Acetamiprid stresses significantly increased and were obviously different from those of the control. When stress was prolonged to six days, the activities of SOD and POD of cucumber seedlings under stresses of three chemical insecticides increased and significantly differed from those of the control. Among the three chemical insecticides, Decamethrin showed greater effects than the other two chemical insecticides. After eight to ten days of stress, the activities of three antioxidant enzymes of cucumber seedlings began to decrease and return to the normal level. Moreover, the contents of MDA and proline in cucumber seedlings under stresses of three chemical insecticides for four to eight days obviously increased compared with those of the control. When cucumber seedlings were stressed respectively by Acetamiprid and Decamethrin for ten days, MDA content became significantly higher than the control, while proline content declined to the

关 键 词：黄瓜幼苗 化学杀虫剂 生物杀虫剂 抗氧化酶 丙二醛 脯氨酸 蛋白质 

分 类 号：S565.4[农业科学—作物学]