北黄海冬季沉积物中线虫个体干质量初探  

Investigation on the individual dry weight of nematodes in sediments of the northern Yellow Sea in winter

作　　者：于婷婷[1,2] 徐奎栋[1] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国科学院大学,北京100049

出　　处：《海洋科学》2015年第6期8-14,共7页Marine Sciences

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-EW-Z-5);国家重点基础研究发展计划项目(2011CB403604)

摘　　要：对冬季采自北黄海海域4个站位沉积物中的线虫进行了分选和制片，通过对总计955条线虫的实测分析，利用体积换算法，获得4个站位线虫的个体平均干质量分别为0．091μg±O．13lμg（O．006-O．545μg）、O．450／μg±O．928μg（0．011-5．078μg）、O．211μg±O．385／ag（0．005～3．093μg）和0．127μg±O．218μg（0．006～2．023μg），4个站位平均为0．196μg±0．140μg，个体干质量最大值是最小值的1028倍。该平均值与自长江口邻近海域所获结果接近，但较南黄海的略低。由此，在利用31μm孔径网筛分选时，中国目前普遍使用的O．4Pg／个经验系数估算的线虫生物量将比实测值高一倍。Pearson相关分析显示，线虫的个体平均干质量与各项环境因子均未见有相关性。造成各站位线虫个体干质量差异的主要因素是线虫的种类组成和幼体所占比例，沉积物粒度、有机质含量、叶绿素a含量等可能是影响种类组成的主要因子，而季节则是影响线虫幼体比例的主要因子。因此，对线虫生物量的估计当以实测为最佳，如采用经验系数则建议采用0．2μg／个进行估算。然而，这一系数是否具有普遍意义尚需进一步验证。Sediment samples collected from four stations in the northern Yellow Sea in winter were sorted and the nema- todes were prepared for measurements. Based on the size measurements of 955 nematodes, we obtained the average indi- vidual dry weight of 0.091μg±0.131μg （0.006-0.545μg）, 0.450μg±0.928μg （0.011-5.078μg）, 0.211μg±0.385μg （0.005±3.093μg） and 0.127μg±0.218μg （0.006-2.023μg） at the four stations, respectively. The overall average individual dry weight was 0.196μg±0.140 μg, with the maximum value about 1028 times of the minimum. This average value is very close to those obtained from the Yangtze Estuary and adjacent sea area, but slightly lower than that from the southern Yellow Sea. Accordingly, calculation using the value of 0.4μg/ind will overestimate the actual biomass of nematodes about one time higher than of the directly measured values when the meiofauna and nematodes were sorted with a 31 μm sieve. Pear- son correlation analysis showed that the average individual dry weight of nematodes did not correlate with any environ- mental parameters. The species composition and juvenile proportion are the main factors regulating the variability of the average individual dry weight of nematodes among stations. Sediment granularity, organic matter and Chlorophyll-a con- tents might be the main parameters influencing species composition, while season is the main factor affecting juvenile pro- portion. For better estimation of nematode biomass, it is suggested to calculate nematode biomass by direct measurement, while simple estimation using 0.2μg/ind rather than 0.4μg/ind is practical for the estimation of nematodes in this sea area. However, whether the index can be generalized needs further investigation.

关 键 词：线虫 个体干质量 实测 生物量估计 北黄海 

分 类 号：P735[天文地球—海洋生物学]