藏药螃蟹甲苯乙醇苷对急性高原肺水肿大鼠的保护作用  

Protective effect of phenylethanoid glycosides extracted from Phlomis younghusbandii on acute high altitude pulmonary edema in rats

作　　者：栾飞[1,2] 李茂星[1] 周保柱[3] 曹馨元[3] 赵一[1] 王先敏[4] 

机构地区：[1]兰州军区兰州总医院药剂科、全军高原环境损伤防治重点实验室,兰州730050 [2]甘肃中医药大学药学院,兰州730000 [3]兰州大学药学院,兰州730000 [4]兰州理工大学石油化工学院,兰州730050

出　　处：《解放军医学杂志》2015年第9期704-709,共6页Medical Journal of Chinese People's Liberation Army

基　　金：全军后勤科研“十二五”计划重点项目(BWS12J012)~~

摘　　要：目的研究藏药螃蟹甲中苯乙醇苷类成分(Ph GCs)对急性高原肺水肿模型大鼠的保护作用。方法 70只大鼠按体重随机分为常压空白组(NG),常压+苯乙醇苷组(NG+Ph GCs,400mg/kg),缺氧模型组(HG),苯乙醇苷高(Ph GCs-H,400mg/kg)、中(Ph GCs-M,200mg/kg)、低(Ph GCs-L,50mg/kg)3个剂量组,地塞米松组(Dex,4mg/kg),每组10只。Ph GCs预防灌胃给药3d,第4天起除常压空白组外,其余各组均置于模拟海拔8000m的高原环境,连续缺氧暴露72h建立实验性高原肺水肿动物模型,然后处死大鼠,干湿比重法检测肺组织含水量,HE染色后光镜下观察大鼠肺组织病理学改变,分光光度法检测大鼠肺组织中SOD、GSH酶的活性及MDA含量,ELISA法检测大鼠血清及肺组织中IL-1β、TNF-α的含量。结果与NG组比较,NG+Ph GCs组大鼠肺组织含水量未见明显变化,HG组大鼠肺组织含水量明显增加(P<0.05)。与NG组比较,HG组大鼠肺组织充血水肿,大量炎症细胞浸润,肺泡间隔及肺泡壁明显增宽;SOD、GSH酶活性明显降低(P<0.05),而IL-1β、TNF-α、MDA含量明显增加(P<0.05)。与HG组比较,Ph GCs各剂量组大鼠肺组织含水量明显降低,炎症细胞浸润、肺泡间隔及肺泡壁增宽程度减轻,SOD、GSH酶活性增加,而IL-1β、TNF-α、MDA含量明显降低(P<0.05)。结论螃蟹甲所含的Ph GCs可明显改善由急性缺氧所致大鼠急性肺损伤的氧化应激和炎症反应,对急性高原肺水肿模型大鼠具有一定保护作用。Objective To investigate the protective effect of phenylethanoid glycosides（Ph GCs） extracted from Phlomis younghusbandii on acute high altitude pulmonar y edema（HAPE） in rats. Methods Seventy male Wistar rats were randomly divided into 7 groups（10 each）： normoxia control group（NG, distilled water）, normoxia＋Ph GCs group（NG＋Ph GCs, 400mg/kg）, hypoxia model group（HG, distilled water）, dexamethasone（Dex, 4mg/kg）, and three groups of Ph GCs in high（Ph GCs-H, 400mg/kg）, middle（Ph GCs-M, 200mg/kg） and low（Ph GCs-L, 50mg/kg） dosage. Ph GCs extracted from Phlomis younghusbandiiwere administered prophylactically to rats for 3 days, and HAPE was induced by the hypobaric hypoxia exposure for 72 hours in an animal decompression chamber with the chamber pressure of 267 mm Hg to simulate an altitude of 8000 m. The water content of rat lung tissue was determined with wet/dry specific gravity method, and histopathologic changes were observed with HE staining. The enzymatic activities of SOD and GSH, and the contents of MDA in lung tissue were determined with spectrophotometry, and the contents of IL-1β, TNF-α in serum and lung tissue were determined with ELISA. Results Compared with NG group, no obvious difference was found in the water content of rat lung tissue in NG＋Ph GCs group, but the water content increased obviously in HG group（P〈0.05）. Compared with NG group, lung tissue congestion and edema were found in HG group, with a heavy inflammatory cell infiltration, widening of alveolar interval, and thickening of alveolar wall were found. The enzymatic activities of SOD and GSH were notablely depressed, and the contents of MDA in lung tissue, and the contents of IL-1β and TNF-α in serum and lung tissue remarkably increased（P〈0.05）. Compared with HG group, the water content of rat lung tissue in each Ph GCs group decreased obviously. Inflammatory cells infiltration, widening of alveolar interval, and thickening of alveolar wall, the enzymatic activity of SOD and

关 键 词：螃蟹甲 苯乙醇苷 高原病 肺水肿 炎症 氧化性应激 

分 类 号：R541.63[医药卫生—心血管疾病]