左归降糖解郁方对糖尿病并发抑郁症大鼠海马星形胶质细胞GFAP、S100B的影响  

Protective - effects of salvianic acid A on ionizing irradiated mice

作　　者：王宇红[1,2] 谭小雯 赵洪庆 杨蕙[3] 孟盼 韩远山[3] 谭元生[3] 

机构地区：[1]湖南省中药粉体与创新药物省部共建国家重点实验室培育基地,湖南长沙410007 [2]湖南省中医药研究院杨永华名老中医药专家传承工作室,湖南长沙410007 [3]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《时珍国医国药》2015年第8期1808-1811,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81373578);湖南省自然科学基金(No.13JJ5030);湖南省科技厅计划项目(No.2012SK3141)

摘　　要：目的研究左归降糖解郁方对糖尿病并发抑郁症大鼠海马星形胶质细胞GFAP及S100B的影响。方法采用高脂乳剂灌胃、尾静脉注射链脲佐菌素(STZ,38mg/kg)、28天慢性应激联合的方法复制糖尿病并发抑郁症大鼠模型,并随机分为5组:模型组,阳性药(二甲双胍0.18g/kg+百忧解1.8mg/kg)组,左归降糖解郁方高、中、低剂量(32.82,16.41,8.20g/kg)组,以正常大鼠为空白组,灌胃给药4周。采用Morris水迷宫测试大鼠空间学习记忆能力,免疫组化法检测海马星形胶质细胞GFAP及S100B的表达情况,Elisa法检测血清中S100B含量。结果与空白组比较,模型组大鼠逃避潜伏期延长,差异有统计学意义(P<0.01);与模型组比较,阳性药组和左归降糖解郁方高剂量组逃避潜伏期缩短,差异有统计学意义(P<0.01)。与空白组比较,GFAP、S100B表达在模型组显著升高,差异有统计学意义(P<0.05);与模型组比较,阳性药组和左归降糖解郁方高剂量组GFAP、S100B表达显著减少(P<0.01或P<0.05)。结论左归降糖解郁方能显著改善大鼠空间学习记忆功能,可能与减少星形胶质细胞反应性增生有关。Objective To investigate the protective effects of salvianic acid A on radiation - induced injury of mice. Methods - BALB/c mice were divided into 5 groups：normal saline control group, radiation saline control,radiation ＆ salvianic acid A 10mg/ kg/day group, radiation ＆ salvianic acid A 20mg/kg/day group and positive control estriol group. Evaluation of the experimental result included the general condition of the mice, survival rate, changes in body weight, RBC, WBC, HGB and PLT counts, spleen weights, spleen index, CFU - spleen. Results 1. Salvianic acid A could protect radiation - induced mice injury and prolong life of the mice obviously. 2. Pretreatment with salvianic acid A significantly increased the RBC, WBC, HGB and PLT counts in irradiated mice. 3. Spleen indexes and CFU - spleen were greatly higher in salvianic acid A pretreated group than others. Conclusion - Salvianic acid A has satisfactory protective effect on radiation - induced mice injury ; the mechanism may be related to the attenuation of hemopoietic system damage.

关 键 词：左归降糖解郁方 糖尿病并发抑郁症 GFAP S100B 

分 类 号：R818.8[医药卫生—放射医学]