超高效液相色谱法同时测定黄芩提取物多指标成分的含量  

Research Scutellaria extract multi-index components UPLC simultaneous determination

作　　者：刘征辉[1,2,3] 魏静娜[1,2] 赵琳琳[1,2] 郭永泽 程奕 

机构地区：[1]天津海世达检测技术有限公司,天津300381 [2]天津市现代中药质量检验中心,天津300381 [3]天津市农业质量标准与检测技术研究所,天津300381

出　　处：《时珍国医国药》2015年第8期1847-1849,共3页Lishizhen Medicine and Materia Medica Research

基　　金："十二五"国家科技支撑计划项目(No.2011BAI07B04)

摘　　要：目的建立黄芩提取物中4种有效成分（黄芩苷、汉黄芩苷、黄芩素和汉黄芩素）同时定量的方法。方法采用超高效液相色谱以ACQUITY UPLCTMBEH C18色谱柱（1.7μm,100×2.1mm）,乙腈-0.1%甲酸溶液进行梯度洗脱,测定波长280 nm,柱温30℃,流速0.2 ml/min。结果 4种成分的线性关系良好,精密度、稳定性、重复性的RSD均低于3.0%,加样回收率为96.2%-99.6%。结论该方法具有简便、灵敏、准确的特点,适用于产品质量的控制。Objective To establish Scutellaria extracts of four active ingredients（ baicalin,wogonoside,baicalein,wogonin） by simultaneous quantitative methods. Methods The chromatographic separation was achieved on a ACQUITY UPLCTMBEH C18column（ 1. 7μm,2. 1 × 100mm）,the mobile phase was Acetonitrile-0. 1% formic acid aqueous solution with gradient elution at a flow rate of 0. 2 ml / min,the detection wavelength was set at 280 nm,and the column temperature was 30℃. Results It showed a good linear relationship of four components,precision,stability,repeatability RSD were less than 3. 0%,recovery was 96. 2% ~99. 6%. Conclusion The method is simple,sensitive,accurate and can be used for quality control Scutellaria extracts.

关 键 词：超高效液相色谱法 黄芩提取物 黄芩苷 汉黄芩苷 黄芩素 汉黄芩素 

分 类 号：R284.1[医药卫生—中药学]