本事琥珀散拆方及其协同作用对子宫内膜异位症大鼠异位内膜侵袭机制影响  

Effects of Benshi Hupo San's Decompositions and Synergistic Effect on Aggression Mechanism in Endometriosis Model Rats

作　　者：刘丹彤[1] 张小勇[1] 马小娜[2] 王冬[1] 杨学琴[1] 王庆国[1] 

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学第三附属医院,北京100029

出　　处：《辽宁中医药大学学报》2015年第9期33-35,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：北京中医药大学自主选题项目(2014-JYBZZ-JS-001)

摘　　要：目的:通过比较本事琥珀散按行气、活血、温经功效拆方及其协同作用,对子宫内膜异位症(EMs)大鼠异位内膜基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶抑制因子-2(TIMP-2)的影响,探讨行气、活血、温经在EMs异位内膜侵袭环节的作用机制。方法:采用自体内膜移植法建立EMs大鼠模型,随机分为模型组、全方组、行气组、活血组、温经补血组、行气活血组、行气温经组、活血温经组和西药对照组,给药4周后免疫印迹(western blot)法同步检测EMs大鼠异位内膜MMP-2和TIMP-2水平。结果:与模型组比较,本事琥珀散组拆方及其协同作用各组异位内膜MMP-2和TIMP-2含量均明显降低,行气活血组异位内膜TIMP-2含量明显降低,甚至低于本事琥珀散全方组,差异具有统计学意义(P<0.05)。结论:本事琥珀散中的行气、活血、温经功效均能够独立抑制异位内膜对基底膜的侵袭,但行气活血协同作用却有利于异位内膜的侵袭生长,不利于其对EMs的治疗,须与温经药物配伍才能起到良好的治疗作用。Objective：Prescripting Benshi Hupo San（BSHPS）according to three different functions as operating qi,activating blood circulation and warming meridian. By comparing the changes of MMP-2 and TIMP-2 in endometriosis model rats by the three functions,we discuss about the Effects of these three functiongs on aggression part to ectopic endometrium in endometriosis. Methods：First,establish the rat model of EMs by transplanting autologous endometrium to the abdominal wall. Then divide the rats randomly into 9 groups：model group,BSHPS group,operating Qi group,activating blood circulation group,warming meridian group,operating Qi and activating blood circulation group,operating Qi and warming meridian group,activating blood circulation and warming meridian group and western medicine control group. Finally,detect the level of MMP-2 and TIMP-2 in ectopic endometrium by western blot,after dosing for 4 weeks. Results：Compared with the model group,the levels of MMP-2 and TIMP-2 in all groups are significantly reduced. The level of TIMP-2 in operating Qi and activating blood circulation group are reduced than the level in BSHPS group（P〈0.05）. Conclusion：The functions of operating Qi,activating blood circulation and warming meridian can separately inhibit the ectopic endometrial invasion and in EMs. The combination of operating Qi and activating blood will promote the planting and invasion of ectopic endometrium,and must be combined with warming meridian and enriching the blood medicine in order to play an obvious role of treating EMs.

关 键 词：本事琥珀散 拆方 子宫内膜异位症 基质金属蛋白酶-2 基质金属蛋白酶抑制因子-2 侵袭机制 

分 类 号：R711.71[医药卫生—妇产科学]