不同居群箭叶淫羊藿基于淫羊藿多苷成分的质量评价  

Quality evaluation of Epimedium sagittatum in different populations based on icariin contents

作　　者：许瑛[1] 石慧君[1] 刘少雄[1] 徐艳琴[1] 

机构地区：[1]江西中医药大学药学院,江西南昌330004

出　　处：《中草药》2015年第15期2284-2290,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31100146;31360036);江西省青年科学家(井冈之星)培养对象计划(20133BCB23024);江西省教育厅科技项目(GJJ13597);江西省卫生厅项目(2012A147);江西省科技专项项目(20123BBG70199)

摘　　要：目的采用HPLC法对箭叶淫羊藿Epimedium sagittatum 16个居群的花期样本进行测定,以期系统全面地评价箭叶淫羊藿的质量,为其种质筛选和资源利用提供参考。方法采用Zorbax SB-C8（250 mm×4.6 mm,5μm）色谱柱,流动相为乙腈-1.44%醋酸水溶液,体积流量1.0 m L/min,柱温25℃,检测波长272 nm。结果居群间质量差异显著,朝藿定A、朝藿定B、朝藿定C和淫羊藿苷的变异范围分别为1.00-16.64、1.00-17.21、1.00-76.21、1.00-46.19 mg/g。从淫羊藿苷的量来看,仅5个居群（HBHF、HBLT、HNLS、HNZJ和JXWN）达到《中国药典》2010年版标准,4个居群低于标准,7个居群未检测到。结论不同居群箭叶淫羊藿药材质量的稳定性和均一性均不理想,但HNZJ、HBLT、HBHF和HNLS居群质量优异,可作为规范化栽培的优异候选种质,同时,其生境也可为栽培基地的选择提供参考。Objective In order to evaluate the quality of Epimedium sagittatum and to provide the references for screening germplasm as well as for the utilization of resources.The concentration variations of main flavonoids,epimedins A-C,and icariin among 15 representative populations of E.sagittatum and one population of E.sagittatum var.glabratum were assessed by HPLC.Methods The chromatographic separation was performed on a Zorbax SB-C8 column（250 mm × 4.6 mm,5 μm）.The mobile phase was a mixture of acetonitrile-1.44%acetic acid aqueous solution in gradient elution.The flow rate was 1.0 mL/min,the detection wavelength was 272 nm,and the column temperature was 25℃.Results Remarkable variations within and among the populations were observed in epimedin A（1.00-16.64 mg/g）,epimedin B（1.00-17.21 mg/g）,epimedin C（1.00-76.21 mg/g）,and icariin（1.00-46.19 mg/g）.As far as the content of icariin concerned,only five populations（HBHF,HBLT,HNLS,HNZJ,and JXWN） had acceptable quality recorded in Chinese Pharmacopoeia,four populations were lower than the standard,and seven populations were even lower than the detectability.Conclusion The present study suggests that E.sagittatum should not be recorded in Chiese Pharmacopoeia indiscriminately,but the populations of HNZJ,HBLT,HBHF,and HNLS are outstanding and excellent,which were the best candidates for germplasm selection.Meanwhile,their habitat can also provide the reference for cultivation.

关 键 词：箭叶淫羊藿 居群 朝藿定A 朝藿定B 朝藿定C 质量评价 种质筛选 

分 类 号：R286.6[医药卫生—中药学]