FQ-PCR检测BALF中结核杆菌-DNA对肺结核诊断的应用研究  

The Application of Mycobacterium Tuberculosis-DNA in BALF by FQ-PCR for the Diagnosis of Pulmonary

作　　者：童建林[1] 饶常红[1] 黄渤[1] 

机构地区：[1]九江学院附属医院,江西九江332000

出　　处：《中国医学创新》2015年第26期66-67,共2页Medical Innovation of China

摘　　要：目的:对FQ-PCR检测BALF中结核杆菌-DNA对肺结核诊断的应用进行研究。方法:随机选取2013年12月-2014年11月期间本院收治的肺结核患者110例作为本次研究的对象,所有患者实施纤支镜检查、活检、刷检,BALF中结核杆菌-DNA行FQ-PCR检测,对检查结果进行分析研究。结果:在本次研究中,确诊为肺结核的患者有40.00%(44/110);FQ-PC检测的敏感性明显高于常规涂片镜检,差异有统计学意义(P<0.05)。结论:在肺结核的诊断中,FQ-PCR检测的敏感度高,操作简单方便,检测速度较快,是BALF中结核杆菌-DNA检查的重要方法,在肺结核的诊断中有重要的应用价值。Objective： To study the application of mycobacterium tuberculosis-DNA in BALF by FQ-PCR for the diagnosis of pulmonary.Method： 110 cases with tuberculosis in our hospital were randomly selected from December 2013 to November 2014 as the objects of the study, all patients were given the fiberoptic bronchoscopy, biopsy, brush biopsy, mycobacterium tuberculosis DNA in bronchoalveolar lavage fluid （ BALF ） was detected by FQ-PCR, the examination results were analyzed. Result： In this study, 44 cases of pulmonary tuberculosis, accounted for the proportion to 40.00% （ 44/110 ） ; the sensitivity of FQ-PC detection was significantly higher than that of conventional smear microscopy, the difference was statistically significant （ P〈0.05 ） .Conclusion： In the diagnosis of pulmonary tuberculosis, the FQ-PCR detection has high sensitivity, simple operation and the detection speed faster, it is an important examination method of mycobacterium tuberculosis DNA in BALF and has important application value.

关 键 词：FQ—PCR检测 BALF 结核杆菌-DNA 肺结核诊断 

分 类 号：R521[医药卫生—内科学] R440[医药卫生—临床医学]