何首乌毛状根培养及其活性成分的产生  

Hairy-root Culture of Polygonium multiflorum Thunb.and the Production of Its Active Constituents-anthraquinones

作　　者：王莉[1] 于荣敏[1] 张辉[1] 程克棣[2] 

机构地区：[1]沈阳药科大学生物技术研究室,沈阳110016 [2]中国医学科学院药物研究所生物合成实验室,北京100050

出　　处：《生物工程学报》2002年第1期69-73,共5页Chinese Journal of Biotechnology

摘　　要：利用发根农杆菌LBA940 2诱导药用植物何首乌产生毛状根。PCR扩增和Southern印迹杂交实验证实发根农杆菌中Ri质粒的T DNA片段已整合进入植物核基因组中。经过基本培养基的筛选和毛状根生长动力学的考察 ,确立了何首乌毛状根在MS培养基中的最佳继代时间为 30d左右。HPLC实验测定结果显示 ,毛状根培养物中大黄酸的含量是原植物的 2Transformed hairy roots of \%Polygonium multiflorum\% Thunb.were obtained by the transformation of \%Agrobacterium rhizogenes\% LBA9402.It was clearly demonstrated that T-DNA of \%A.Rhizogenes\% Ri plasmid was integrated into the cells of hairy roots by the experiments of PCR and Southern hybridization.Through the screen of basic medium and the research of growth curve of hairy roots,the optimum inoculum time was selected in 30 days or so in the optimum condition-MS medium.On the condition,the content of rhein was 2.85 folds higher in hairy roots than that of natural plants by means of HPLC.

关 键 词：何首乌 毛状根培养 活性成分 发根农杆菌 蒽醌类化合物 诱导 

分 类 号：Q943.1[生物学—植物学]