子痫前期患者胎盘间充质干细胞抑制淋巴细胞增殖的研究  

Placental mesenchymal stem cells from pre-eclampsia patients in suppressing lymphocyte proliferation

作　　者：沙文琼[1] 折瑞莲[1] 柯茹[1] 王硕石[1] 

机构地区：[1]暨南大学第二临床医学院产科,深圳518020

出　　处：《白求恩医学杂志》2015年第4期343-347,F0004,共6页Journal of Bethune Medical Science

基　　金：深圳市科技计划项目(编号:201302031)

摘　　要：目的纯化和体外培养子痫前期患者的胎盘间充质干细胞(p MSCs),研究子痫前期患者p MSCs的免疫抑制能力。方法从子痫前期患者及正常妊娠者胎盘组织中纯化培养p MSCs,应用Transwell板将p MSCs与混合淋巴细胞共培养,检测淋巴细胞数,计算淋巴细胞增殖抑制率。在培养基中加入干扰素γ,分别从mRNA水平和蛋白质水平检测p MSCs的吲哚胺双加氧酶(indoleamine 2,3-dioxygenase,IDO)表达水平。结果来源于子痫前期患者的PMSCs和正常妊娠者的形态相同,表达CD44、CD29、CD73、CD90、CD105、HLA-I类分子,极少表达CD34、CD45、CD14及HLA-Ⅱ分子。能够在体外被诱导分化为成骨细胞及成脂细胞。细胞释放的可溶性分子具有抑制淋巴细胞增殖的作用;细胞表达低水平的IDO,但当受到IFN-γ刺激后,表达水平显著提高(P<0.05)。对照正常妊娠组和子痫前期组p MSCs对淋巴细胞增殖的抑制率和表达IDO水平,差异均不具有显著性(P>0.05)。结论 p MSCs所表达的IDO和释放的可溶性分子可能与PE的发生、发展无关;由于现有体外培养技术不能反映p MSCs在体内的状态,也可能导致原本存在的差异难以体现出来。Objective To isolate and culture placental mesenchymal stem cells （pMSCs）from women with pre-eclampsia and study the immunosuppressive effects of uhese cells. Methods Placental mesenchymal stem ceils isolated from placenta of women with pre-eclampsia and normal pregnancy were cultured in vitro. Co-culture of placental mesenchymal stem cells and mixed lymphocyte were performed on Transwell and the lymphocytes were counted and the inhibiting ratio of lymphocytes proliferation was calculated. Moreo- ver, placental mesenchymal stem cells was treated with interferon γ （IFN-γ）. The expression of Indoleamine 2,3-Dioxygenase （IDO） mRNA and protein of these cells were determined by qRT-PCR and ELISA. Results Placental mesenchymal stem cells from women with preelampsia had the same morphology with normal pregnancy. Most of these cells were positive for CD44, CD29, CD73, CD90, CD105,HLA-I molecules but negative for CD34, CIM5,CD14, HLA-Ⅱ molecules. They could also be induced to adipogenic and os- teogenic differentiation. Soluble factors secreted by these cells may suppress the proliferation of lymphocytes. IFN-γ, treated cells pro- voked significantly more IDO production than the untreated （ P 〈 0.05）. No significant differences were seen between the cells from normal pregnancy and PE, both on suppression of lymphocytes proliferation, as well as expression of IDO, regardless of the IFN-γ stimulation. Conclusion The expression of IDO and soluble factors by placental mesenchymal stem ceils might have no association with the pre-eclampsia pathogenisis. Since in vitro culture conditions can not reflect the actual features of placental mesenchymal stem cells, the difference between normal and pre-eclampsia may not be revealed.

关 键 词：子痫前期 胎盘间充质干细胞 成骨诱导分化 成脂诱导分化 吲哚胺双加氧酶 混合淋巴细胞反应 

分 类 号：R714.244[医药卫生—妇产科学]