窄谱中波紫外线对体外培养黑素细胞增殖及代谢影响研究  

Effects of NB-UVB on Proliferation, Melanogenesis and Tyrosinase Activity in Cultured Human Melanocytes

作　　者：安彩霞[1,2] 赵亚楠[2] 李婷婷[2] 王红娟[2] 普雄明[2] 

机构地区：[1]新疆医科大学,乌鲁木齐830001 [2]新疆维吾尔自治区人民医院,乌鲁木齐830001

出　　处：《中国中西医结合皮肤性病学杂志》2015年第4期231-234,共4页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

摘　　要：目的探讨窄谱中波紫外线(Narrow band ultraviolet B,NB-UVB)对体外培养黑素细胞增殖及黑素代谢的影响。方法不同剂量的NB-UVB作用于体外培养纯的黑素细胞,细胞增殖与活性测定试剂盒(Cell counting kit-8,CCK-8)测定黑素细胞增殖的影响;多巴氧化方法测定酪氨酸酶活性,Na OH裂解法测定黑素含量。结果 NB-UVB对体外培养黑素细胞的增殖有明显抑制作用,且有剂量依赖效应;而对黑素细胞的酪氨酸酶活性及黑素生成有不同程度的促进作用。结论 NB-UVB对黑素细胞的增殖具有抑制作用,而对其功能有促进作用。Objective To study the impacts of NB-UVB on cell proliferation and metabolism in cultured human melanocytes. Methods Melanoeytes was incubated different doses of NB-UVB. Melanoeytes proliferation was detected by CCK-8 method. The activity of tyrosinase was tested by dopa oxidization reaction. Melanogenesis was detected by NaOI-I assay. Results MeIanoeytes proliferation was significantly inhibited by NB-UVB, the effect of NB-UVB on melanocytes was dose-dependent. Tyrosinase activity and melanogenesis was promoted by NB-UVB in varying degrees. Conclusion Melanoeytes proliferation is directly inhibited by NB-UVB, but tyrosinase activity and melanogenesis of melanocytes is promoted by NB-UVB.

关 键 词：窄谱中波紫外线 黑素细胞 影响 

分 类 号：R758.5[医药卫生—皮肤病学与性病学]