东亚钳蝎神经毒素基因BmK IT_3的克隆及其在大肠杆菌中的表达  

Cloning and Expression of Buthus martensiiKarsch Scorpion Toxin Gene (BmK IT_3) in Escherichia coli

作　　者：于继彬[1] 季平[1] 查新民[1] 沈卫德[1] 吴祥甫[2] 

机构地区：[1]苏州大学生命科学院蚕桑系,苏州215151 [2]中国科学院上海生命科学院生物化学与细胞研究所,上海200031

出　　处：《生物工程学报》2002年第1期106-108,共3页Chinese Journal of Biotechnology

基　　金：江苏省自然科学基金项目资助 (No .99KJB180 0 0 1)~~

摘　　要：According to the reported sequence of \%Buthus martensii\% Karsch scorpion toxin gene (BmK IT 3),we synthesized two primers,which were complementary in a region.By the means of PCR,we got the gene.The gene was fused in expression vector pET-28a,which gave rise to a recombinant plasmid pET(IT 3 R).Then it was transformed into \%E.coli\% BL21 (DE 3).With IPTG induction,the gene was efficiently expressed.And the fusion product was soluble.According to the reported sequence of \%Buthus martensii\% Karsch scorpion toxin gene (BmK IT 3),we synthesized two primers,which were complementary in a region.By the means of PCR,we got the gene.The gene was fused in expression vector pET-28a,which gave rise to a recombinant plasmid pET(IT 3 R).Then it was transformed into \%E.coli\% BL21 (DE 3).With IPTG induction,the gene was efficiently expressed.And the fusion product was soluble.

关 键 词：东亚钳蝎 神经毒素基因 BmKIT3 融合表达 大肠杆菌 

分 类 号：Q786[生物学—分子生物学]