DNA生物催化功能研究进展  

Biocatalytic Activity of DNA

作　　者：于乐成[1] 王升启[2] 顾长海[1] 毛青[1] 

机构地区：[1]第三军医大学西南医院全军感染病研究所,重庆400038 [2]军事医学科学院放射医学研究所,北京100850

出　　处：《中国生物化学与分子生物学报》2002年第4期391-397,共7页Chinese Journal of Biochemistry and Molecular Biology

摘　　要：近年来发现 ,不少结构特殊的DNA分子分别具有剪切RNA分子或DNA分子、T4聚核苷酸激酶样活性、DNA连接酶样活性以及催化卟啉金属离子化等多种生物催化功能 ,这些DNA分子被称为脱氧核酶或酶性DNA .它们在用作RNA和DNA工具酶、基因分析和诊断手段以及基因治疗药物等方面的潜力引人注目 .综述这些DNA分子的种类、结构特征。Recently, many special DNA molecules have been found to possess important and interesting biocatalytic activities, such as cleaving RNA or DNA, T4 polynucleotides like activity, DNA ligase like activity and promoting the incorporation of a metal atom into porphyrin (metallation of porphyrin). These catalytic DNA molecules were named as deoxyribozymes,DNA enzymes or DNAzymes. They have great perspective to be used as RNA or DNA treating tools, gene analysis, gene diagnosis, and gene therapy. The latest developments of their clasification,characteristic structure,catalytic activity,current and future application are reviewed.

关 键 词：DNA 生物催化功能 研究进展 脱氧核酶 RNA工具酶 DNA工具酶 基因治疗 

分 类 号：Q523[生物学—生物化学]