丹蛭降糖胶囊对糖尿病大鼠肝脏PI3K/AKT信号通路的影响  

The Influence of “Danzhi Jiangtang Capsule”on the PI3K/AKT signaling pathways in the liver of diabetic rats

作　　者：李中南[1] 方朝晖[1] 熊园园[2] 刘珊珊[2] 张帆[2] 黄平[2] 

机构地区：[1]安徽中医药大学第一附属医院内分泌科,安徽合肥230031 [2]安徽中医药大学研究生部,安徽合肥230031

出　　处：《上海中医药杂志》2015年第9期67-70,共4页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局国家中医临床研究基地业务建设科研专项资助项目(JDZX2012004)

摘　　要：目的探讨丹蛭降糖胶囊(DJC)对糖尿病大鼠肝脏PI3K/AKT信号通路的影响。方法取雄性SD大鼠55只,除正常对照组外,其余动物给予高脂饲料4周,按35μg/g给大鼠腹腔注射链脲佐菌素(STZ)溶液1次。造模成功后,大鼠再分为模型组,DJC高、低剂量组及吡格列酮组。DJC高、低剂量组分别给予DJC 1.08 g·kg-1·d-1、5.04 g·kg-1·d-1,吡格列酮组给予吡格列酮10μg·kg-1·d-1,模型组、正常对照组给予等量0.9%Na Cl溶液。模型组、药物组继续给予高脂饲料8周,并连续灌胃8周,留取肝脏,逆转录反应及实时荧光定量PCR检测大鼠肝脏组织PI3K、AKT mRNA表达,取血清测定血糖。结果与正常对照组比较,模型组大鼠PI3K、AKT mRNA表达明显升高(P<0.01);予DJC后,肝组织PI3K、AKT mRNA表达下降,与模型组比较差异有统计学意义(P<0.01,P<0.05)。大鼠造模后血糖明显升高(P<0.01),DJC灌胃后,高、低两组血糖显著下降(P<0.01),疗效与吡格列酮近似(P>0.05)。血糖与PI3K/AKT相关分析显示,二者呈显著正相关。结论 DJC对糖尿病大鼠肝脏PI3K/AKT信号通路过度表达有显著干预作用,可能与保护肝脏、减轻胰岛素抵抗有关。Objective The aim of this study is to investigate the effect of ＂Danzhi Jiangtang Capsule＂ on PI3K/AKT signaling pathway in the liver of diabetic rats. Methods 55 male SD rats were fed with high calorie diet except the normal control group for 4 weeks. A single intraperitoneal injection of streptozotocin （STZ） of 35mg/kg, when the diabetic rodent model was build, the rats were randomly re-divided into 4 groups： model group, high-does group of DJC, low-does group of DJC and pioglitazone group. High-does group of DJC and low-does group of DJC were treated with 1.08 g/（ kg ·d） and 0.54 g/（ kg · d） repectively, pioglitazone group was treated with 10mg/（ kg · d）, the blank group and the model group were treated with the same volume of saline . Continuous intragastric administration for 8 weeks, took the liver, detected 13-actin, P13K/AKT in liver tissue of rats by reverse transcription reaction and Quantitative Real-time PCR. Total RNA was extracted from the liver tissue of rats with Trizol kit, determinated the blood glucose and blood lipid. Results Expression of PI3K/AKT in model group increased significantly , DJC evidently inhibited the excessive expression of P13 K/AKT and RNA. Compared with the normal control group （P 〈 0.05 or P 〈 0.01 ） ; the blood glucose of rats were increased significantly （ P 〈0.01 ） compared with. After being given ＂Danzhi Jiangtang Capsule ＂ for 8 weeks, the level of glucose was decreased（ P 〈 0.01 ） , curative effect was similar to Pioglitazone. The blood glucose and the PI3K/AKT correlation analysis showed a significant positive correlation. Conclusion The DJC has a significant intervention effect on the over expression of PI3 K/AKT signaling pathway in the liver of diabetic rats, this may be related to the protection of the liver, reduced vascular endothelial inflammatory reaction.

关 键 词：丹蛭降糖胶囊 磷脂酰肌醇3激酶/苏氨酸蛋白激酶(PI3K/AKT) 糖尿病 大鼠 

分 类 号：R285.5[医药卫生—中药学]