正交实验优化甲氨蝶呤囊泡的制备  

Optimizing the preparation of methotrexate niosomes using the orthogonal test

作　　者：张波[1] 王启平 王慧云[3] 全先高[3] 王盈盈[1] 王宁[1] 

机构地区：[1]济宁医学院基础学院,山东济宁272067 [2]济宁任城区卫生监督所,济宁272100 [3]济宁医学院药学院,山东日照276826

出　　处：《济宁医学院学报》2015年第4期247-250,共4页Journal of Jining Medical University

基　　金：济宁市科技局课题资助项目(编号:2009-19)

摘　　要：目的制备甲氨蝶呤囊泡并通过正交实验优化甲氨蝶呤囊泡的制备工艺,探索最适宜的药物包封条件。方法采用L9(34)正交法优化甲氨蝶呤囊泡对药物包封率的影响因素,以囊泡包封率为考察指标,确定最佳制备工艺条件。结果司盘40(Span40)与胆固醇形成的囊泡(VSpan40∶VCHOL=4∶2),在50℃超声40min的条件下,对0.60g/L的甲氨蝶呤包封率最高,可达60%以上。结论通过正交实验优化制得的甲氨蝶呤囊泡可获得较高的包封率。Objective The preparation process of the methotrexate niosomes were optimized by means of orthog- onal experiment to explore the most suitable drug coating conditions. Methods The methotrexate encapsulation rate affecting factors was optimized by an L9 （3^4 ） orthogonal test. The niosomes encapsulation rate as evaluation index was used to determine the best preparation program. Results The results showed that the entrapment efficiency of the niosomes prepared under the condition which were as follows： Span40 and cholesterol （VSpan40： VCHOL）was 2 ： 1,the dosage of methotrexate,Ultrasonic temperature and time were 0.60g/L, 50℃ and 40min, and the entrap- ment rate could reach more than 60%. Conclusion The methotrexate niosomes optimized by orthogonal test had a higher entrapment rate.

关 键 词：甲氨蝶呤 囊泡 包封率 正交实验 

分 类 号：R943[医药卫生—药剂学]