不同产地赤雹根中总皂苷含量的比较分析  

COMPARATIVE ANALYSIS OF TOTAL SAPONINS COTENTS IN THLADIANTHA DUBIA ROOTS FROM DIFFERENT PRODUCING AREA

作　　者：刘春楠 洪文婷[1,2] 祝晴晴 佟继铭[1,2] 刘永平[1,2] 

机构地区：[1]承德医学院中药研究与开发重点实验室 [2]承德医学院中药研究所,河北承德067000

出　　处：《承德医学院学报》2015年第5期371-373,共3页Journal of Chengde Medical University

基　　金：河北省科技厅基金项目(14272502D)

摘　　要：目的:测定不同产地赤雹根中总皂苷的含量,为选择赤雹根种植地域提供依据。方法:采用紫外-可见分光光度法测定三个不同产地赤雹根中总皂苷的含量:以齐墩果酸为对照品,5%香草醛-冰醋酸和高氯酸为显色剂,检测波长为545nm。结果:赤雹根总皂苷在13.13-39.41μg/ml范围内线性关系良好,回归方程为A=0.0559C-0.0271,r=0.9997。总皂苷平均回收率为98.9%,RSD值为1.99。三个不同产地赤雹根药材中总皂苷的含量分别为0.12%(东北吉林、栽培)、0.38%(河北承德、栽培)、0.59%(河北青龙、野生)。结论:三个不同产地赤雹根药材中赤雹根总皂苷含量差异较大,以河北青龙野生赤雹根药材中赤雹根总皂苷含量最高,人工栽培品中以河北承德的含量较高。Objective： To determine the total saponins contents in Thladiantha Dubia Roots from different producing area and provide basis for choosing planting regions of Thladiantha Dubia Roots. Methods： UV spectrophotometry was used to determine the total saponins contents in Thladiantha Dubia Roots from 3 producing areas, with oleanolic acid as reference substance, 5% vanillin-acetic acid and perchloric acid as color development reagent, and detecting wave length was 545 nm. Results： Total saponins in Thladiantha Dubia Roots had good linear relationship at concentration of 13.13-39.41μg/ml. The regression equation was A=0.0559C-0.0271（r=0.9997）. The average recovery rate was 98.9%, RSD was 1.99. The total saponins contents in Thladiantha Dubia Roots from 3 producing areas were respectively 0.12%（Dongbei Jilin, cultivation）, 0.38%（Hebei Chengde, cultivation） and 0.59%（Hebei Qinglong, uncultivated）. Conclusions： The differences between contents of total saponins in Thladiantha Dubia Roots form 3 producing areas are great. The contents of total saponins in uncultivated Thladiantha Dubia Roots form Hebei Qinglong is the highest; For cultivated Thladiantha Dubia Roots, the contents of total saponins form Hebei Chengde is fairly higher.

关 键 词：赤雹根 总皂苷 含量测定 产地 

分 类 号：R917[医药卫生—药物分析学]