高脂高糖饲料联合STZ诱导2型糖尿病大鼠模型STZ最佳剂量探讨  

STUDY ON OPTIMAL DOSE OF STZ IN TYPE 2 DIABETES MELLITUS RATS' MODEL INDUCED BY HIGH-FAT AND HIGH-SUGAR DIET COMBINED STZ

作　　者：肖艳红[1] 谷佳琦[2] 杨晔娟 牛硕莹 王月堂 

机构地区：[1]承德医学院生物化学教研室,河北承德067000 [2]承德医学院

出　　处：《承德医学院学报》2015年第5期376-378,共3页Journal of Chengde Medical University

摘　　要：目的:探讨高脂高糖饲料喂养联合链脲佐菌素(STZ)腹腔注射建立2型糖尿病大鼠模型时STZ的最佳用量。方法:32只Wistar大鼠随机分为4个造模组,给予高脂高糖饲料喂养后腹腔注射不同剂量的STZ(15mg/kg、25mg/kg、35mg/kg、45mg/kg)。分别观察各组大鼠的成模率、死亡率、空腹血糖和体重。结果:造模组Ⅲ(STZ用量35mg/kg)成模率高且死亡率较低;STZ注射72h后,各造模组大鼠空腹血糖水平随STZ注射剂量的增加逐渐升高;造模结束4周后各组大鼠的血糖仍维持在较高水平,且体重明显降低。结论:高脂高糖饲料喂养联合STZ腹腔注射诱导2型糖尿病大鼠模型时STZ的最佳剂量为35mg/kg。Objectives： To explore the optimal dose of streptozocin（STZ） in type 2 diabetes mellitus rats＇ model induced by high-fat and high-sugar diet combined STZ. Methods： 32 Wistar rats were randomly divided into 4 model groups. The rats in each model group were fed with high-fat and high-sugar diet and then injected different dose of STZ into the peritoneal cavity respectively（dose of STZ： 15mg/kg, 25mg/kg, 35mg/kg, 45mg/kg）. The successful rate, death rate, fasting blood glucose and body weight of rats in each group were observed. Results： The successful rate of model group Ⅲ（35mg/kg） was fairly higher but death rate was fairly lower. 72 h after injecting STZ, the fasting blood glucose of rats in each model group gradually increased with increasing of STZ dosage. 4 weeks after successfully established type 2 diabetes mellitus rats＇ model, the blood glucose of rats in each model group also remained at a high level, but the body weight of rats decreased obviously. Conclusions： The optimal dose of STZ when induced type 2 diabetes mellitus rats＇ model by using high-fat and high-sugar diet combined STZ is 35mg/kg.

关 键 词：2型糖尿病 动物模型 STZ注射量 

分 类 号：R945[医药卫生—微生物与生化药学]