控制减压治疗兔重型颅脑外伤后海人藻受体表达的实验研究  

Expression of kainic receptor in rabbits with severe head trauma treated by controlled decompression: an experimental research

作　　者：蔡显锋[1] 王玉海[1] 何新俊[1] 陈卫良[1] 蔡桑[1] 杨理坤[1] 陈磊[1] 

机构地区：[1]解放军第101医院神经外科,江苏无锡214044

出　　处：《中华神经外科疾病研究杂志》2015年第4期323-326,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：南京军区十一五重点课题基金资助项目(09Z002)

摘　　要：目的通过比较常规开颅减压和控制减压两种不同手术减压方式对治疗兔重型颅脑外伤后急性颅内高压脑组织中海人藻受体亚基5(GluR5)和海人藻受体亚基6(GluR6)的表达水平及脑组织微观病理变化,探讨控制减压技术治疗重型颅脑伤的可能机制,为控制减压手术方法在临床应用提供理论依据。方法采用硬膜外球囊加压方法制备兔重型颅脑外伤后急性颅内高压的动物模型,新西兰白兔随机分为假手术组、控制减压组和常规减压组,观察术后24 h GluR5和GluR6的表达情况、术后兔脑组织HE染色、术后24 h ICP变化情况及术后兔头颅CT的变化。结果术后24 h,控制减压组GluR5的表达明显高于常规减压组(P<0.05),GluR6的表达明显低于常规减压组(P<0.05);脑组织HE染色控制减压组明显轻于常规减压组;控制减压组术后24 h ICP值明显低于常规减压组(P<0.05);控制减压组的脑梗塞发生率(10.5%)明显低于常规减压组(35.2%)(P<0.05)。结论控制减压模式治疗兔重型颅脑外伤能有效减轻脑缺血再灌注损伤(cerebral ischemia-reperfusion injury,I/R),降低术后脑梗塞等并发症发生率,起到明显的脑保护作用。Objective The expressions of the kainic recepors （ GluR5 and GluR6 ） and the pathological changes of the brain tissues in rabbits with severe head trauma were compared between conventional decompression and controlled decompression groups to provide the experimental basis for the application of controlled decompression in clinical practices.Methods The animal model of rabbit with severe head trauma was established by epidural balloon compression method.New Zealand white rabbits were randomly divided into sham operation group, routine decompression group and controlled decompression group.The expressions of GluR5 and GluR6, HE staining of brain tissues, and the changes of skull CT 24 h post-operation were observed.Results At 24 h post-operation, the expression of GluR5 of controlled decompression group was significantly higher than that of conventional decompression group;the expression of GluR6 of conventional decompression group was obviously more than that of the controlled decompression group.HE staining of brain tissues of controlled decompression group was significantly better than that of conventional decompression group.ICP at 24 h post-operation in control pressure group was significantly lower than that of conventional decompression group.The incidence of cerebral infarction in the controlled decompression group （10.5%） was significantly lower than that of the conventional decompression group （35.2%）.Conclusion Controlled decompression operation can effectively alleviate ischemia-reperfusion injury of brain tissues in rabbits with severe head trauma and reduce the post-operative complications, such as cerebral infarction, so it plays a protective role on brain tissues.

关 键 词：控制减压 海人藻受体 海人藻受体亚基5 海人藻受体亚基6 缺血再灌注损伤 颅脑外伤 

分 类 号：R741[医药卫生—神经病学与精神病学]