通心络对糖尿病足大鼠内皮功能的影响  

The effects of Tongxinluo capsule on the treatment of endothelial function in diabetic foot rats

作　　者：高怀林[1] 贺会仙[1] 孔青[1] 王宏涛 曹月香[1] 丁来标[1] 李会玉[1] 孙永辉[1] 许辉[1] 张建波[1] 

机构地区：[1]河北以岭医院内分泌科,石家庄050091 [2]河北以岭医药研究院新药研发中心,石家庄050035

出　　处：《疑难病杂志》2015年第9期888-890,894,F0003,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：河北省中医药管理局科学研究计划项目(No.2012112;2014082)

摘　　要：目的探讨通心络对糖尿病足大鼠内皮细胞功能指标[一氧化氮(NO)、内皮素(ET)、血管性假血友病因子(vWF)],内皮细胞的形态及ET、内皮型一氧化氮合酶(eNOS)、诱生型一氧化氮合酶(iNOS)表达的影响及其可能机制。方法健康SD大鼠45只,分为对照组、模型组及通络组,各15只。采用高脂喂养、链脲佐菌素(STZ)和结扎并剪断SD大鼠股动脉和股静脉的方法复制糖尿病足大鼠模型,对照组、模型组给予以生理盐水灌胃,通络组给予通心络治疗,共6周。生化法检测血清NO、ET、vWF水平,光镜、透射电镜观察血管内皮细胞的形态和超微结构变化,免疫组化观察ET1、eNOS、iNOS的表达。结果与对照组比较,模型组NO水平降低,ET、vWF水平升高(P<0.05,P<0.01);与模型组比较,通络组糖尿病足大鼠血清NO增加、血浆ET、vWF降低(P<0.05,P<0,01),内皮细胞形态有明显改善,eNOS表达增加,ET、iNOS表达减少。结论通心络治疗能改善糖尿病足大鼠血管内皮细胞的损伤和功能障碍。Objective To investigate the effect of Tongxinluo capsule on diabetic foot rat endothelial function index：nitric oxide （ NO） , endothelin （ ET） , vascular von Willebrand factor （ vWF） and endothelial cell morphology and ET ,endo-thelial nitric oxide synthase （eNOS） and inducible nitric oxide synthase （iNOS）, and its possible mechanism.Methods Forty-five healthy SD rats were divided into control group , model group with 15 rats in each group.high-fat feeding, streptozo-tocin （ STZ） , ligation and cut the femoral artery and femoral vein of SD rat to establish diabetic foot rat model , control group , model group were given normal saline gavage , Tongluo group was given Tongxinluo capsule treatment for 6 weeks.Serum lev-els of NO, ET, and vWF were detected by biochemical method .The morphology and ultrastructure of vascular endothelial cells were observed by light microscope and transmission electron microscope .The expression of eNOS , iNOS and ET were ob-served by immunohistochemistry .Results Compared to the control group , model group ’ s NO level was decreased , ET and vWF levels were elevated （ P 〈0.05, P 〈0.01）;compared with the model group , Tongluo group diabetic foot rats ’ level of NO was increased, plasma ET and vWF were decreased （ P 〈0.05, P 〈0.01）, the morphology of endothelial cells were sig-nificantly improved , and eNOS expression was increased , ET and iNOS expression was reduced .Conclusion Tongxinluo capsule can improve diabetic foot rat vascular endothelial cell from injury and dysfunction .

关 键 词：通心络 糖尿病足 内皮功能 大鼠 

分 类 号：R285[医药卫生—中药学]