清脑复神液精制工艺再评价研究  

Re-evaluation on purification process of Qingnao Fushen Oral Liquid

作　　者：刘涛[1] 伍利华[2] 李鹏程[1] 刘婷[1] 徐小艳[1] 伍月[1] 徐玉玲[1] 

机构地区：[1]成都大学,四川成都610106 [2]广西中医药大学,广西南宁530001

出　　处：《中草药》2015年第16期2407-2412,共6页Chinese Traditional and Herbal Drugs

基　　金：四川省经信委产业技术研究与开发资金项目([2014]1124号);四川省科技厅科技支撑计划项目(2014SZ0139)

摘　　要：目的对清脑复神液精制工艺进行再评价研究,为改进该制剂的生产工艺提供实验依据。方法以盐酸小檗碱转移率、总黄酮转移率、固含物降低率为指标,采用正交试验优选活性炭吸附工艺;以盐酸小檗碱转移率、总黄酮转移率、固含物降低率为指标,考察陶瓷膜孔径大小、滤过压力、滤过温度以及滤液收集量;同时采用不同的清洗剂组合筛选较优的膜清洗方法。结果活性炭吸附工艺为活性炭用量为0.1%、温度为常温、吸附时间20 min;滤过工艺条件为陶瓷膜孔径为200 nm的微滤膜、压力0.10 MPa、温度40℃、滤液收集量为原体积的1.25倍;膜清洗方法为先采用Na OH循环冲洗30 min,再用HNO3循环冲洗30 min。结论陶瓷膜分离技术用于精制清脑复神液效果较好,可产业化应用。Objective To reevaluate the purification process of Qingnao Fushen Oral Liquid（QFOL）. Methods The orthogonal experiment of adsorption process of the activated carbon was optimized with the diversion rates of berberine and total flavonoids and the reduction of solid content as indexes. The aperture, pressure, temperature, and collection were optimized with the diversion rates of berberine and total flavonoids and the reduction of solid content as indexes. At the same time, the method of membrane cleaning was screened through the combination of different cleaners. Results The adsorption process of activated carbon was as following： the usage of activated carbon was 0.1%, at normal atmospheric temperature, and the adsorption time was 20 min. The optimal process was aperture 200 nm, pressure 0.10 MPa, temperature 40 ℃, and collection 1.25 times of the original volume. The cleaning method of membrane is to use Na OH to clean for 30 min and HNO3 to clean for 30 min. Conclusion The purified QFOL can be obtained by this technique, which is helpful for industrialization.

关 键 词：清脑复神液 预处理 膜分离技术 小檗碱 总黄酮 

分 类 号：R284.2[医药卫生—中药学]