胶艾汤对血虚模型大鼠补血作用的有效部位筛选  

Screening of effective parts in Jiaoai Decoction with blood enriching function in blood deficiency model rats

作　　者：贾梅[1] 郑传柱[1] 张丽[1] 丁安伟[1] 

机构地区：[1]南京中医药大学江苏省方剂高技术研究重点实验室/江苏省中药资源产业化过程协同创新中心,江苏南京210023

出　　处：《中草药》2015年第16期2428-2433,共6页Chinese Traditional and Herbal Drugs

基　　金：江苏省高校自然科学研究项目(12KJA360002);江苏高校优势学科建设工程资助项目(ysxk-2014)

摘　　要：目的比较胶艾汤不同溶剂提取部位对血虚模型大鼠外周血象、免疫器官指数、白细胞介素2(IL-2)和促红细胞生成素(EPO)水平以及红细胞膜能量代谢酶活性的影响,筛选胶艾汤补血作用的有效部位。方法采取每天于眼底静脉丛放血5 mL/kg,连续放血12d的方法,复制大鼠血虚模型;造模同时各组动物分別ig给予胶艾汤及其正丁醉、石油醚、醋酸乙酯、水部位浸膏(剂量均为生药量12g/kg),以复方阿胶浆为阳性对照。造模第12天检测各组大鼠外周血红细胞计数(RBC)、血红蛋白(HGB)、红细胞压积(HCT)、血小板计数(PLT),计算脾脏和胸腺器官指数。检测血清中IL-2和EPO水平,检测全血红细胞膜能量代谢酶Na^+,K^+-ATP及Ca^(2+),Mg^(2+)-ATP酶活性,UPLC法初步分析胶艾汤不同提取部位的化学成分差异。结果与对照组比较,模型组大鼠RBC、HGB、HCT、PLT、胸腺指数以及IL-2水平明显降低,Na^+,K^+-ATP酶和Ca^(2+),Mg^(2+)-ATP酶活性明显减弱,脾脏指数及EPO水平明显升高,表明血虚模型复制成功。与模型组比较,胶艾汤正丁醇组可明显升高HCT、RBC、HGB、PLT(P<0.05、0.01);降低脾脏指数及EPO水平(P<0.05、0.01);明显升高胸腺指数、IL-2水平(P<0.01),升高红细胞膜Ca^(2+),Mg^(2+)-ATP酶及Na^+,K^+-ATP酶活性(P<0.05、0.01)。石油醚组可明显升高PLT及IL-2水平(P<0.01),降低EPO水平及脾脏指数(P<0.05、0.01)。醋酸乙酯组可明显降低脾脏指数及EPO水平(P<0.01),升高红细胞膜Ca^(2+),Mg^(2+)-ATP酶活性(P<0.05)。水部位组可明显升高PLT(P<0.05),降低EPO水平(P<0.01)。胶艾汤正丁醇提取部位中检测到其他提取部位不含有的咖啡酸、苯甲酰芍药苷、甘草酸铵,且正丁醇提取部位中没食子酸、原儿茶酸、磷酸川芎嗪、绿原酸的量均高于胶艾汤醋酸乙酯和水提取部位。结论正丁醇部位是胶艾汤补血作用的最强有效部位。Objective To screen the active parts with enriching the blood effects of different solvent extraction parts from Jiaoai Decoction（JAD） by comparing the peripheral blood,immune organ index,interleukin 2（IL-2）.and erythropoietin（EPO） and the effect of content of erythrocyte membrane and energy metabolism enzyme activity of blood deficiency model rats.Methods Taking the fundus venous plexus blood 5 mL/kg every day,continuous bleeding for 12 d,to establish the rat model of blood deficiency;The model animals in each group were ig given JAD and n-butanol,petroleum ether,ethyl acetate,parts of water extract（12 g/kg crude drug） with compound E-Jiao slurry as a positive control.On day 12 of modeling,the erythrocyte count（RBC）,hemoglobin（HGB）,red blood cell hematocrit（HCT）,platelet count（PLT） were detected in rats and immune organ indexes of spleen and thymus were calculated,the levels of IL-2 and EPO content were detected,energy metabolism enzymes（Na^＋,K^＋-ATPase and Ca^2＋,Mg^2＋-ATPase） activity were investigated;The difference of the chemical constituents in the different solvent extraction of JAD was analyzed by UPLC.Results Compared with the control group,the RBC.HGB,HCT,PLT,thymus index,and IL-2 levels of rats in the model group decreased significantly,Na^＋,K^＋-ATPase and Ca^2＋,Mg^2＋-ATPase activity weakened significantly,the spleen index and EPO levels were significantly incrcased,indicating that blood deficiency model was successfully established.Compared with the model group,HCT,RBC,HGB,and PLT（P〈 0.05,0.01） of rats in n-butyl alcohol group could be significantly increased;The spleen index and the content of EPO（P〈 0.05,0.01） were reduced;The thymus index and the content of IL-2（P0.01） were increased,the erythrocyte Ca^2＋,Mg^2＋-ATPase and Na^＋,K^＋-ATPase activity（P〈 0.05,0.01） was elevated.The contents of PLT and IL-2（P〈 0.01） in petroleum ether group could be obviously increased;The content of EPO and the spleen index（P〈 0.

关 键 词：胶艾汤 补血作用 部位筛选 促红细胞生成素 NA^+ K^+-ATP酶 CA^2+ MG^2+-ATP酶 

分 类 号：R285.5[医药卫生—中药学]