蛋白AAX14401相应抗体的制备及鉴定  

The antibody preparation of protein AAX14401 and its determination

作　　者：胡少华[1] 赵振理[2] 孙光[3] 畅继武[3] 

机构地区：[1]山西医科大学第二临床医学院,山西太原030001 [2]山西医科大学第二医院,山西太原030001 [3]天津市泌尿外科研究所,天津120000

出　　处：《临床医药实践》2015年第9期683-685,共3页Proceeding of Clinical Medicine

基　　金：国家自然科学基金资助项目(项目编号:30371601)

摘　　要：目的：由AY887902完整读码框翻译的蛋白AAX14401,制备蛋白AAX14401相应抗体anti-AY,并对其进行鉴定。方法：以AY887902的ORF所翻译氨基酸与GSTP1相比差异较大的40∽52位上氨基酸序列为模板,按照抗体制备要求,合成多肽并免疫小鼠,制备蛋白AAX14401多克隆抗体anti-AY。结果：经过用酶联免疫吸附剂测定法（ELISA）检测,抗体滴度为1︰35 000,滴度达到要求,可以进行蛋白AAX14401的检测。结论：制备的蛋白AAX14401抗体anti-AY符合要求,为进一步对AY887902功能进行检测奠定了较好的基础。Objective： According to AAX14401,which is translated by AY887902 integrity reading frame,preparation of antibody named anti-AY to AAX14401,and determinate the anti-AY. Methods： Amino acid translated by ORF of AY887902 showed significant deviations of GSTP1 at amino acid sequence 40 to 52 which we used as template,then according to the demand of preparation of antibodies,synthetic polypeptide and inject into mouse to make its polyclonal antibody： anti-AY. Results： Enzyme-linked immunosorbent assay（ ELISA） results reviewed that titer of the polyclonal is 1 ︰ 35 000,which suggested that the polyclonal antibody was eligibility to detect AAX14401. Conclusion： The antibody of anti-AY prepared of protein AAX14401 appropriate for the demand,and make a well foundation for detect the functional of AY887902.

关 键 词：抗体制备 突变体 膀胱肿瘤 

分 类 号：R97[医药卫生—药品]