AB-8大孔吸附树脂同时分离纯化茶树根总黄酮、总皂苷工艺研究  

Study on the Purification Process of the Total Flavonoids and Total Saponins of the Tea Root by Macroporous Resin AB-8

作　　者：余雄英[1,2] 周军[1] 曹树稳[2] 欧阳永伟[1] 

机构地区：[1]江西中医药大学,江西南昌330004 [2]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《现代中药研究与实践》2015年第4期46-49,共4页Research and Practice on Chinese Medicines

基　　金：江西中医药大学2012年重点学科教师培养计划资助项目:茶树根抗痛风活性成分的筛选研究(2012jzzdxk018)

摘　　要：目的研究AB-8大孔吸附树脂纯化茶树根总黄酮、总皂苷的工艺条件。方法以总黄酮、总皂苷的吸附率和转移率为指标,考察吸附药液质量浓度、药液p H、药材树脂比、洗脱乙醇体积分数、乙醇洗脱用量等影响因素。结果吸附药液质量浓度0.3 g·ml-1,药液p H=6,药材树脂比1:2,依次用12BV水洗,10 BV 30%乙醇洗脱得总黄酮,10 BV 70%乙醇洗脱得总皂苷。结论采用大孔吸附树脂AB-8可较好地纯化茶树根总黄酮与总皂苷,纯化后茶树根总黄酮含量、总皂苷含量均达60%以上。Objective To discuss purification technology conditions of total flavonoids and total saponins from tea root by AB-8 macroporous resin.Methods With absorption rate and metastasis rate of total flavonoids and total saponins as indexes,investigated concentration of sample liquid, p H, ratio of macroporous resin to crude drug, concentration of eluted ethanol and other factors. Results Optimum purification conditions were as follows： Concentration of sample liquid was 0.3 g · ml-1, p H of sample liquid was 6,ratio of crude drug to macroporous resin was1 ： 2, total flavonoids and total saponins were eluted by 12 BV water,10 BV 30% ethanol and 10 BV 70% ethanol respectively. Conclusion Macroporous resin AB-8 could be used to purify total flavonoids and total saponins from tea root,the content of total flavonoid and total saponins reached more than 60%.

关 键 词：AB-8大孔吸附树脂 茶树根 总黄酮 总皂苷 纯化工艺 

分 类 号：R284.2[医药卫生—中药学]