具有谷胱甘肽过氧化物酶活性的小分子模拟物2-TeCD保护线粒体抵抗氧化损伤  

Protection of Mitochondria Against Oxidative Damage by the GPX Mimic 2-TeCD

作　　者：薛雁[1] 任晓君[1] 张鲲[1] 牟颖[1] 刘俊秋[1] 曹鹏 郭灿辉[1] 罗贵民[1] 沈家骢[2] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,长春市130023 [2]吉林大学超分子结构与材料教育部重点实验室,长春市130023

出　　处：《中国生物化学与分子生物学报》2002年第4期506-510,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：973项目 (G2 0 0 0 0 7810 2 );国家自然科学基金资助项目 (No .2 0 0 72 0 10 )

摘　　要：首次合成了一个新的含碲化合物 2 TeCD(二碲桥联环糊精 ) ,并证实其具有谷胱甘肽过氧化物酶 (GPX)活力 ,能清除氢过氧化物 .与其它GPX模拟物相比 ,2 TeCD具有分子量小 ,水溶性好及良好的化学和生物学稳定性 ,且其GPX活力比另一种被广泛认知的小分子模拟物Ebselen高约 4 6倍 .用Fe2 + Vc诱导的线粒体损伤体系研究了 2 TeCD的抗氧化性质 ,结果发现在相同剂量下 2A novel dicyclodextrinyl ditelluride (2\|TeCD) compound was devised to mimic GPX and its activity towards H\-2O\-2 was found to be 46\^7 U/μmol, which had activity 46 times more than ebselen, a well\|studied GPX mimic. Antioxidant activity was studied by using ferrous sulfate/ascorbate\|induced mitochondria damage model system. In order to determine the capacity for 2\|TeCD to antioxidize, the swelling, lipid peroxidation products and cytochrome c oxidase activity of mitochondria were chosen as standards. 2\|TeCD decreased the swelling of mitochondria, inhibited the lipid peroxidation, retained the activity of cytochrome\|c oxidase and exhibited great antioxidant ability in comparison with ebselen.

关 键 词：谷胱甘肽过氧化物酶活性 小分子模拟物 2-TeCD 线粒体 抗氧化损伤 

分 类 号：Q554[生物学—生物化学]