米诺环素抑制大鼠脊髓背角胶状质神经元的超极化激活电流  

Minocycline reduces hyperpolarization- activated current in rat substantia gelatinosa neurons

作　　者：朱梦叶 刘娜娜[2] 彭斯聪 李凌超[1] 张达颖[1] 柳涛[2] 

机构地区：[1]南昌大学第一附属医院疼痛科,江西南昌330006 [2]南昌大学第一附属医院儿科,江西南昌330006

出　　处：《南方医科大学学报》2015年第8期1155-1161,共7页Journal of Southern Medical University

基　　金：国家自然科学基金(81260175);江西省教育厅科技项目(GJJ13122)~~

摘　　要：目的 研究米诺环素对大鼠脊髓背角胶状质（SG）神经元超极化激活电流（Ih）的影响。方法选取3～5周龄雄性SD大鼠，制作离体脊髓横切片，应用全细胞膜片钳技术记录SG神经元矗，灌流不同浓度米诺环素（1-300μmol／L）并观察其对厶的影响。结果记录的SG神经元中约50％可记录到Ih，该电流被，Ih阻断剂CsCl和ZD7288阻断。米诺环素可减低川唇值、减小Ih电流密度，此效应呈可逆性及剂量依赖性，半数有效抑制浓度（IC50）为34μmol／L。结论米诺环素具有抑制脊髓背角SG神经元Ih的作用，从而降低SG神经元兴奋性，对疼痛的调控具有重要的意义。Objective To investigate the effect of minocycline on hyperpolarization-activated current （Ih） in the substantia gelatinosa （SG） neurons in rat spinal dorsal horn. Methods In vitro spinal cord transverse slices were prepared from 3-5-week-old male Sprague-Dawley rats. Using whole-cell patch clamp technique, Ih currents were recorded before and after bath application of minocycline （1-300μmol/L） to the SG neurons. Results Ih currents were observed in nearly 50% of the recorded neurons, and were blocked by Ih blocker CsCl and ZD7288. Minocycline rapidly and reversibly reduced the amplitude of Ih and decreased the current density in a concentration-dependent manner with an ICs0 of 34 μmol/L. Conclusion Minocycline suppresses the excitability of SG neurons through inhibiting the amplitude and current density of Ih and thereby contributes to pain modulation.

关 键 词：米诺环素 超极化激活电流 脊髓背角胶状质神经元 

分 类 号：R965[医药卫生—药理学]