Wnt7a-Akt/mTORa信号通路在肾衰营养胶囊改善慢性肾衰竭大鼠骨骼肌萎缩中的作用  

Shenshuai Yingyang capsule ameliorates muscle atrophy in rats with chronic renal failure:role of Wnt7a-Akt/m TOR signal pathway

作　　者：王明[1] 汪东涛 尹懿 鲁路 石莹[1] 黄燕峰 陈德秀 魏连波[1,2] 

机构地区：[1]南方医科大学珠江医院中西医结合肾病中心,广东广州510282 [2]中医药学院,广东广州510515

出　　处：《南方医科大学学报》2015年第8期1170-1174,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81173457,81373808,81403215)~~

摘　　要：目的探讨Wnt7a-Akt/m TOR信号通路在肾衰营养胶囊改善慢性肾衰竭(CRF)大鼠骨骼肌萎缩中的作用及机制。方法采用切除5/6肾的方法制作CRF模型,予4%酪蛋白饲料喂养制作CRF营养不良大鼠模型。将大鼠随机分为正常组、模型组、中药组和开同组。采用组织病理学检测TA横截面积并计算肌纤维面积分布;采用同位素14+C-苯丙氨酸掺入法检测肌肉蛋白合成;采用Western blot检测Wnt7a-Akt/m TOR信号通路中的蛋白表达。结果肾衰营养胶囊增加了CRF大鼠体质量和骨骼肌质量的作用,形态学表现为TA横截面积增加,促进蛋白质合成增加以及骨骼肌组织Wnt7a-Akt/m TOR信号通路中蛋白表达上调。结论肾衰营养胶囊具有改善CRF模型大鼠骨骼肌萎缩的作用,其机制可能上调骨骼肌Wnt7a-Akt/m TOR信号通路和促进骨骼肌蛋白质合成相关。Objective To observe the effect of Shenshuai Yingyang Capsule （SSYYJN） in ameliorating muscle atrophy in rats with chronic renal failure （CRF） and explore the role of Wnt7a-Akt/mTOR signal pathway in mediating this effect. Methods Male rats were randomly assigned to 5/6 nephrectomy group and sham-operated group, and the former group was further randomly divided into CRF model group, KA group, and SSYY]N group. The size of anterior tibia muscle was examined microscopically with HE staining. Protein synthesis in the soleus muscle was investigated by ＂C-phenylalanine experiment, and the expression of Wnt7a, frizzled-7, phospho-Akt, phospho-mTOR and GAPDH were detected with Western blotting. Results The body weight, the wet and dry weight, cross-sectional area, and muscle protein synthesis of the anterior tibia muscles, and expressions of the proteins in the Wnt7a/Akt signaling pathway all increased significantly in SSYYJN and KA groups as compared with those in the model group. Conclusion SSYYJN can effectively improve muscle atrophy in the rat model of CRF possibly by reversing the reduction in the expressions of Wnt7a/Akt signaling pathway proteins in the skeletal muscles.

关 键 词：慢性肾衰竭 营养不良 骨骼肌萎缩 Wnt7a 肾衰营养胶囊 

分 类 号：R692.5[医药卫生—泌尿科学]