胆固醇酯转移蛋白转基因家兔制作及生物学特性分析  

Establishment of cholesteryl ester transfer protein transgenic rabbits by DNA microinjection and analysis of their biological properties

作　　者：高守翠 成大欣[1] 赵四海[1,2] 陈玉龙[1] 王晓靖[1] 白亮[1] 范江霖[1,3] 刘恩岐[1,2] 

机构地区：[1]西安交通大学心血管研究中心脂代谢与动脉粥样硬化研究室,西安710061 [2]西安交通大学医学院实验动物中心,西安710061 [3]山梨大学医学部分子病理学系

出　　处：《中国实验动物学报》2015年第4期331-335,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(No.81370379;81070250)

摘　　要：目的制作肝脏特异性高表达人胆固醇酯转移蛋白(CETP)转基因家兔并对其生物学特性分析进行分析。方法利用显微注射方法制作CETP转基因家兔,利用Western blot、real-time PCR和CETP活性鉴定试剂盒鉴定模型家兔中人CETP转基因的表达和活性。结果 PCR检验结果显示成功获得CETP转基因家兔,转基因主要在肝脏特异性表达,其血浆CETP活性相对于非转基因家兔明显升高。结论成功建立了高表达人CETP的转基因家兔,为研究CETP功能和其参与心血管疾病的机制提供了良好模型。Objective The aim of this study was to generate human cholesteryl ester transfer protein（ CETP）transgenic rabbits and analyze their biological properties. Methods We generated human CETP transgenic rabbits by DNA microinjection,and detected the expression of human CETP by real-time PCR and Western blot assay. The activity of CETP was measured using an activity assay kit. Results Human CETP transgenic rabbits were successfully generated by DNA microinjection. Compared with wide type rabbits,the expression of human CETP was dramatically increased in the liver of the human CETP transgenic rabbits. The plasma CETP activity was also much higher in the liver of human CETP transgenic rabbits than that of control rabbits. Conclusions The model of human CETP transgenic rabbits is successfully established by DNA microinjection. It will provide a useful tool for the studies of CETP biological function and its involvement in the mechanisms of cardiovascular diseases.

关 键 词：胆固醇酯转移蛋白 转基因 家兔 血浆 肝 

分 类 号：Q95-33[生物学—动物学]