黄芪多糖干预环磷酰胺所致免疫抑制小鼠的免疫功能  

Enhancing effect of astragalus polysaccharide on the immune function in cytoxan-induced immunosuppressed mice

作　　者：张芮琪 陈正礼[1] 罗启慧[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014

出　　处：《中国实验动物学报》2015年第4期389-394,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家科技支撑计划课题(2014BAI03B01);国家重大科学仪器设备开发专项(2013YQ49085906);四川省青年科技创新研究团队项目(2013TD0015)

摘　　要：目的探讨黄芪多糖对免疫抑制小鼠免疫功能的调节作用,并建立一套可行的多糖类物质免疫调控指标与评价体系。方法运用黄芪多糖(astragalus polysaccharide,APS)分别处理正常及环磷酰胺(Cytoxan,CTX)致免疫抑制模型小鼠,通过测定其胸腺和脾脏指数、腹腔巨噬细胞吞噬率和吞噬指数,并对小鼠胸腺和脾脏的组织发育情况进行观察;结合流式细胞术检测了各组小鼠外周血T淋巴细胞亚群(CD3+、CD4+和CD8+)百分含量的变化。结果黄芪多糖能显著促进正常小鼠及免疫抑制小鼠的免疫器官增重,增大其脾脏和胸腺器官指数;组织学观察结果也显示黄芪多糖可以促进正常小鼠脾脏和胸腺的组织发育,并能改善CTX所致的小鼠脾脏和胸腺组织发育损伤。黄芪多糖可提高正常小鼠及免疫抑制小鼠腹腔巨噬细胞的吞噬百分率及吞噬指数,并可明显提高正常及免疫抑制小鼠外周血血清中CD3+、CD4+和CD4+/CD8+比值,降低CD8+百分含量(P<0.05或P<0.01)。结论黄芪多糖可提高小鼠机体的免疫机能,并建立了较为全面的多糖类物质免疫调控评价指标。Objective To explore the regulatory effect of astragalus polysaccharide（ APS） on immune function in immunosuppressed mice,and to establish a set of feasible indexes of immunological regulation and evaluation system of polysaccharides. Methods Both Cytoxan（ CTX）-induced immunosuppressed mice and normal mice were treated with astragalus polysaccharide（ APS）. Spleen index,thymus index,phagocytosis rate and phagocytic index of peritoneal macrophages were assessed,and the spleen and thymus tissues were examined by histology. The percentage of CD3＋,CD4＋,and CD8＋cells in peripheral blood were determined by flow cytometry. Results APS significantly increased the thymus and spleen indexes in both immunosuppressed mice and normal mice,promoted the histological development of spleen and thymus in normal mice,enhanced the recovery of histological structures in the spleen and thymus in the CTX-induced immunosuppressed mice. APS also enhanced the phagocytosis rate and phagocytic index of peritoneal macrophages in both immunosuppressed mice and normal mice. In addition,the percentage of CD3＋and CD4＋cells,the ratio of CD4＋/ CD8＋in peripheral blood of both CTX-induced immunosuppressed mice and normal mice were increased while CD8＋cells were decreased. Conclusions The results of this study suggest that astragalus polysaccharide can improve the non-specific immunity and cellular immunity in mice,and indicate that we established comprehensive evaluation indexes of immunoregulatory effects of polysaccharides.

关 键 词：黄芪多糖 小鼠 环磷酰胺 免疫功能 

分 类 号：Q95-33[生物学—动物学]