金银花指纹图谱及其清除DPPH自由基的谱-效关系  

Chromatography-efficacy Relation Between HPLC Fingerprints of Lonicerae Japonicae Flos and Its Effect in Scavenging DPPH Free Radicals

作　　者：梁从莲 刘红燕[1] 崔媛[1] 王玲娜[1] 孙希芳[1] 张永清[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《中国实验方剂学杂志》2015年第17期44-46,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2011BAI06B01);山东省自主创新专项(2013CXC20401)

摘　　要：目的:探讨金银花清除DPPH自由基的活性成分。方法:采用HPLC获得金银花甲醇提取液指纹图谱,并对色谱图进行融合处理;同时建立提取液对DPPH自由基的清除方程,采用双变量相关分析将DPPH自由基的半数清除率IC50与HPLC指纹图谱共有峰的标准化峰面积比值关联。结果:4(绿原酸),10(异绿绿原酸B),12(异绿原酸C)号共有峰含量变化与DPPH自由基清除活性显著负性相关(P<0.05)。结论:金银花液相指纹图谱与清除DPPH自由基的谱效关系,对于金银花药用物质基础研究具有一定参考价值。Objective： To investigate the active components in Lonicerae Japonicae Flos for scavenging DPPH free radicals. Method： The HPLC fingerprints were developed and merged. Meanwhile,the DPPH radical scavenging equation was established. The bivariate correlation analysis was applied to correlate the scavenging DPPH Free radical parameter IC50 and the standardized peak area ratio of common peaks of HPLC fingerprints.Result： The changes in the contents of common peaks 4（ chlorogenic acid）,10（ isochlorogenic acid B） and 12（ isochlorogenic acid C） show significant negative correlation with DPPH free radical scavenging activity.Conclusion： The chromatography-efficacy relation between the HPLC fingerprints and the DPPH free radical scavenging activity provides a certain reference value for the basic study on medicinal material base of Lonicerae Japonicae Flos.

关 键 词：金银花 指纹图谱 DPPH自由基清除 谱-效相关 

分 类 号：R284.1[医药卫生—中药学]