HPLC同时测定清热解毒胶囊中6种成分的含量  

Simultaneous Determination of Six Effective Components in Qingre Jiedu Capsules by HPLC

作　　者：林远凤[1] 黄燕萍[2] 

机构地区：[1]广西北海市人民医院,广西北海536000 [2]广西北海食品药品检验所,广西北海536000

出　　处：《中国实验方剂学杂志》2015年第17期67-70,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的：建立HPLC测定清热解毒胶囊中绿原酸、栀子苷、木犀草苷、黄芩苷、连翘苷和汉黄芩素含量的方法。方法：采用Eclipse XDB色谱柱（4.6 mm×150 mm,5μm）,流动相乙腈-0.4%磷酸溶液,梯度洗脱,流速0.80 m L·min-1,检测波长275nm。绿原酸、栀子苷、木犀草苷、黄芩苷、连翘苷和汉黄芩素与其相邻质谱峰能完全分离。结果：绿原酸、栀子苷、木犀草苷、黄芩苷、连翘苷和汉黄芩素的线性范围分别为7.83-313 mg·L-1（r=0.999 9）,4.30-132 mg·L-1（r=0.999 8）,0.836-33.5 mg·L-1（r=0.999 7）,4.36-349 mg·L-1（r=1.000 0）,0.731-58.4 mg·L-1（r=0.999 7）,0.314-12.6 mg·L-1（r=0.999 7）。方法回收率均不低于98%。结论：该方法简便、准确、重复性好,能排除其他成分的干扰,可用于该制剂的质量控制的评价。Objective： A HPLC method was established for the determination of chlorogenic acid,geniposide,luteolin,baicalin,phillyrin and wogonin in Qingrejiedu capsules. Method： Eclipse XDB column（ 4. 6 mm × 150 mm,5 μm） was adopted and eluted in gradient with the mobile phase of acetonitrile-0. 4%phosphoric acid solution. The flow rate was 0. 80 m L ·min- 1. The detection wavelength was set at 275 nm.Chlorogenic acid, geniposide, luteolin, baicalin, phillyrin and wogonin could be completely separated from adjacent mass spectra peaks. Result： The HPLC method showed a good linear range of 7. 83-313 mg·L- 1（ r =0. 999 9） for chlorogenic acid,4. 30-132 mg·L- 1（ r = 0. 999 8） for geniposide,0. 836-33. 5 mg·L- 1（ r =0. 999 7） for luteolin,4. 36-349 mg·L- 1（ r = 1. 000 0） for baicalin,0. 731-58. 4 mg·L- 1（ r = 0. 999 7） for phillyrin and 0. 314-12. 6 mg·L- 1（ r = 0. 999 7） for wogonin,with the recoveries above 98%. Conclusion： The method is simple,accurate and highly reproducible and so can be used to remove interferences from other components and evaluate the quality control of the preparation.

关 键 词：高效液相色谱 绿原酸 栀子苷 木犀草苷 黄芩苷 连翘苷 汉黄芩素 清热解毒胶囊 

分 类 号：R284.1[医药卫生—中药学]