脾虚四号方干预对功能性腹泻脾虚证模型大鼠肠上皮细胞微绒毛形态的影响  

Effect of Pixu Ⅳ Recipe on Morphology of Intestinal Epithelial Microvillus in Functional Diarrhea Spleen Deficiency Syndrome Model Rats

作　　者：康楠[1] 王凤云[2] 陈婷[2] 王晓鸽[2] 朱恩林[1] 唐旭东[2] 

机构地区：[1]北京中医药大学,北京100029 [2]中国中医科学院西苑医院,北京100091

出　　处：《中国实验方剂学杂志》2015年第17期79-83,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)项目(2013CB531703)

摘　　要：目的:从微观结构的角度观察脾虚四号方干预大鼠腹泻模型肠上皮细胞微绒毛形态的变化。方法:健康雄性Wistar大鼠36只随机分为正常组、模型组、脾虚四号方高、中、低剂量组(4.6,2.3,1.2 g·kg-1)及阳性药思密达组(0.9 g·kg-1)6组,采用水环境小平台站立法复合高乳糖饲料喂养的方法复制腹泻模型,模型成功后,ig给药7 d,取空肠、回肠、结肠组织,通过透射电镜技术观察脾虚四号方对大鼠腹泻模型肠上皮细胞微绒毛微观结构的影响。结果:正常组微绒毛排列整齐划一,粗细均匀;模型组空肠黏膜微绒毛变化较小;回肠黏膜微绒毛的变化开始明显,2.5万倍电镜下即可看到微绒毛的排列普遍不整齐,长短不一,电子密度不均匀;近端结肠,远端结肠则黏膜损害逐渐加重,尤以远端结肠最为明显,微绒毛排列紊乱,长短不一,部分缺如,微绒毛变细,形态不规整,且伴有黏膜下分泌小泡增多。给予脾虚四号方及阳性药干预后,均有不同程度改善,尤以脾虚四号方低剂量组最为明显,几乎与正常组无差异;其次是高、中剂量组与阳性药组差异不明显。结论:水环境小平台站立法复合高乳糖饲料喂养的方法复制的腹泻模型,其空肠黏膜上皮细胞微绒毛形态变化不明显,从回肠到近端结肠再到远端结肠,微绒毛形态变化逐渐明显,破坏程度逐渐增加,而脾虚四号方低剂量组对其改善最为明显,其次为高剂量组。Objective： To observe the changes in intestinal epithelial microvillus of functional diarrhea spleen deficiency symptom model rats after the treatment with Pixu Ⅳ recipe. Method： Totally 36 male healthy rats were divided into the normal group,the model group,Pixu Ⅳ recipe high,medium and low doses groups（ 4. 6,2. 3,1. 2 g·kg- 1） and the positive Smecta（ 0. 9 g·kg- 1） group. The high fat sucrose diets and the water environment small platform standing method were adopted to establish the diarrhea model. After the successful modeling,the rats were orally given drugs for 7 days. The transmission electron microscopy was used to observe the effect of Pixu Ⅳ recipe on the microstructure in intestinal epithelial microvillus. Result： The normal group showed neat and even microvilli. The model group showed small changes in microvilli of the jejunum mucous membrane;significant changes in microvilli of ileum mucous membrane,with uneven microvilli of ileum mucous membrane and low electron density under electron microscope（ × 25 000）, serious damages in proximal and distal colons,particularly uneven,thin and irregular microvilli and more secreted vesicles under mucous membrane. All of the above phenomena were relieved to varying extent after the intervention with Pixu Ⅳ recipe and positive drugs. The Pixu Ⅳ recipe low dose group showed the most significant relieve,which was close to the normal group; the PixuⅣ recipe high dose group was next to it,whereas the Pixu Ⅳ recipe medium dose group and the positive drug group showed no significant difference. Conclusion： The diarrhea model established with the high fat sucrose diets and the water environment small platform standing method shows no significant morphologic change in epithelial cell microvilli of jejunal mucosa and gradually obvious morphologic changes and damage degree from ileum,proximal colon to distal colon,the changes became more and more serious. Pixu Ⅳ recipe can relive the microstructure changes of the microvilli in the diar

关 键 词：脾虚四号方 脾虚证 功能性腹泻 微绒毛 

分 类 号：R285.5[医药卫生—中药学]