蝙蝠葛多酚类生物碱对心肌细胞动作电位的影响  

Effect of Phenolic Alkaloids of Menispermum dauricum on Cardiac Electrophysiology

作　　者：邢莎莎[1] 金肆[2] 许高[2] 李文婧[2] 龚培力[2] 杨晓燕[2] 

机构地区：[1]成都大学附属医院,成都610081 [2]华中科技大学同济医学院,武汉430030

出　　处：《中国实验方剂学杂志》2015年第17期88-91,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2012ZX09303018-001);成都大学校青年基金项目(2015XJZ10)

摘　　要：目的:研究蝙蝠葛多酚类生物碱(phenolic alkaloids of Menispermum dauricum,PAMD)对豚鼠乳头肌和兔窦房结优势起搏细胞动作电位的影响。方法:运用玻璃微电极记录豚鼠心室乳头肌动作电位,研究不同浓度PMAD(1,3,10μmol·L-1)对心室乳头肌动作电位时程(action potential duration,APD),有效不应期(effective refractory period,ERP),最大除极化速度(maximum upstroke velocity,Vmax),动作电位幅度(amplitude,APA),超射(overshoot,OS)的影响;运用玻璃微电极记录兔窦房结优势起搏细胞动作电位,研究不同浓度PAMD(1,3,10μmol·L-1)对窦房结细胞APD、最大舒张期电位(maximal diastolic potential,MDP),APA,Vmax,自发放电频率(rate of pacemaker firing,RPF)和舒张期去极化速度[velocity of diastolic(phase 4)depolarization,VDD]的影响。结果:在豚鼠右心室心肌乳头肌细胞中研究发现,PAMD可以剂量依赖性的显著延长APD和ERP,延缓Vmax。当浓度为10μmol·L-1时,PAMD对窦房结自律性具有轻度影响,这种作用与对照组比有显著差异。结论:PAMD对心室肌的作用强于其对窦房结的作用,这表明其具有成为理想的抗室性心律失常的药物的潜力。Objective： To study the effect of phenolic alkaloids of Menispermum dauricum（ PAMD） on the action potential in papillary muscles of guinea pig and dominant pacemaker cells of rabbit sinoatrial nodes.Method： Capillary grass microelectrode was used to record the paction potential in papillary muscles of guinea pigs,study the effect of different concentrations（ 1,3,10 μmol·L- 1） of PAMD on action potential duration（ APD）,effective refractory period（ ERP）,maximum upstroke velocity（ Vmax）,amplitude（ APA） and overshoot（ OS）. Capillary grass microelectrode was also used to record the paction potential in dominant pacemaker cells of rabbit sinoatrial nodes and study the effect of different concentrations（ 1,3,10 μmol·L- 1） of PAMD on APD,maximal diastolic potential（ MDP）,APA,Vmax,rate of pacemaker firing（ RPF）,and velocity of diastolic（ phase4） depolarization（ VDD）. Result： According to the study for papillary muscle cells of right ventricular myocardia,PAMD can significantly prolong APD and ERP and delay Vmaxin a concentration-dependent manner.PAMD also had a mild effect on the automatic rhythmicity of sinus nodes at concentration of 10 μmol·L- 1,with a significant difference with the control group. Conclusion： PAMD have a stronger effect on ventricular muscles than sinoatrial nodes,suggesting that it has the potential to be prepared into an ideal drug for ventricular arrhythmia.

关 键 词：蝙蝠噶多酚类生物碱 心室乳头肌 窦房结细胞 动作电位 

分 类 号：R285.5[医药卫生—中药学]