几种强心苷类成分的镇痛活性  

Analgesic Activity of Several Cardiac Glycosides

作　　者：邓璐璐[1] 张佳佳[1] 谢海琴[1] 张楠[1] 陈霞[1] 叶兰[2] 郝小江[1] 张援虎[1] 

机构地区：[1]贵州省中国科学院天然产物化学重点实验室,贵阳550002 [2]贵阳医学院药理学教研室,贵阳550004

出　　处：《中国实验方剂学杂志》2015年第17期116-120,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省中药现代化项目(黔科合中药字[2011]5085号);贵州省高层次人才科研条件特助项目(TZJF-2006-35)

摘　　要：目的:探讨了华蟾毒精、远华蟾毒精、沙蟾毒精和杠柳苷4种强心苷类成分的镇痛作用及华蟾毒精的镇痛作用机制。方法:昆明种小鼠按要求分为空白组,阿司匹林阳性组(200 mg·kg-1),各受试化合物高、中、低剂量组,采用小鼠热板法和醋酸扭体法观察4种强心苷类化合物的镇痛作用;通过注射盐酸纳洛酮(2 mg·kg-1)和外周阿片受体阻断剂(纳洛酮四价盐衍生物,NAL-M,20 mg·kg-1)初步研究华蟾毒精的镇痛作用机制。结果:与空白组比较,华蟾毒精、远华蟾毒精、沙蟾毒精和杠柳苷均能提高热板所致小鼠舔足的痛阈(P<0.05,P<0.01),也能使醋酸所致小鼠扭体次数减少(P<0.05,P<0.01);ip纳洛酮和NAL-M后,均能拮抗华蟾毒精对热板所致小鼠痛阈值的提高作用(P<0.05,P<0.01)。结论:华蟾毒精、远华蟾毒精、沙蟾毒精和杠柳苷均具有镇痛作用,且华蟾毒精的镇痛作用与外周阿片受体有一定的关联。Objective： To investigate the analgesic activity of four cardiac glycosides-cinobufagin,telocinobufagin,arenobufagin and periplocin and the analgesic mechanism of cinobufagin. Method： According to the requirements,the KM mice were divided into the blank group,the asprin positive group（ 200 mg·kg- 1）,and groups of high,medium and low dose of test compounds. The analgesic effects of the four cardiac glycosides in mice were observed by the hot-plate test and acetic acid writhing methods. Moreover,the potential analgesic mechanism of cinobufagin was preliminarily investigated by injecting naloxone hydrochloride（ 2 mg·kg- 1） and peripheral opioid receptor antagonist naloxone methiodide（ NAL-M,20 mg·kg- 1）. Result： Compared with the blank group,all of the four cardiac glycosides can increase the pain threshold of paw-licking induced by hot plate（ P〈0. 05,P〈0. 01） and decrease the number of writhing induced by acetic acid in mice（ P〈0. 05,P〈0. 01）. The intraperitoneal injection with naloxone hydrochloride and NAL-M can antagonize the increase in the pain threshold of paw-licking induced by hot plate（ P〈0. 05, P〈0. 01）. Conclusion： Cinobufagin,telocinobufagin,arenobufagin and periplocin have the analgesic effect,and the analgesic effect of cinobufagin may be correlated with the peripheral opioid receptor.

关 键 词：强心苷 镇痛作用 华蟾毒精 杠柳苷 外周阿片受体 

分 类 号：R285.5[医药卫生—中药学]