黄芪及其活性成分黄芪多糖对心衰模型小鼠同型半胱氨酸、核因子-κB和D-二聚体水平的影响  

Effect of Astragali Radix and Its Active Ingredient Astragalus Polysaccharideits on Homocysteine,NF-κB and D-Dimer in Heart Failure Model Mice

作　　者：柴连海 王丽丽[1] 陈文生[1] 

机构地区：[1]唐山市工人医院,河北唐山063000

出　　处：《中国实验方剂学杂志》2015年第17期142-145,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2013年唐山市科学技术研究与发展指导计划项目(13130269b)

摘　　要：目的:探究中药黄芪及其活性成分黄芪多糖对心衰模型小鼠同型半胱氨酸(Hcy),核因子-κB(NF-κB)和D-二聚体(D-D)水平的影响。方法:SPF级雄性小鼠100只,随机选取20只为正常组,另80只ip异丙肾上腺素建立心衰模型,造模成功后分为黄芪组(3 g·kg-1),黄芪多糖组(3 g·kg-1),阳性药组(地高辛,0.05 mg·kg-1)和模型组,每组各20只,黄芪组以3 m L·kg-1·d-1剂量ig黄芪提取物原液,黄芪多糖组按3 g·kg-1·d-1剂量ip,阳性药组以0.05 mg·kg-1·d-1剂量ig地高辛,模型组无菌生理盐水3 m L·kg-1·d-1ig,ip与ig均为日1次,连续干预4周后,取其心尖部心肌组织进行病理学检查,腹主动脉取血进行Hcy,NF-κB和D-D水平的检测。结果:与正常组比较,模型组Hcy,NF-κB和D-D水平明显升高(P<0.05),病理结果显示模型组心肌纤维数量减少,染色变浅,横纹模糊,部分坏死灶被瘢痕组织代替;与模型组比较,各给药组Hcy,NF-κB和D-D水平明显降低(P<0.05),病理结果显示各给药组心尖部心肌组织坏死病灶明显减轻;与黄芪组及地高辛组比较,黄芪多糖组效果更明显。结论:中药黄芪及其活性成分黄芪多糖对心衰模型小鼠Hcy,NF-κB和D-D有明显的降低作用,但后者的作用效果好于前者。Objective： To explore the effect of Astragali Mongolici Radix and its active ingredient Astragali Mongolici Radix polysaccharideits on the expressions of homocysteine（ Hcy）,nuclear factor（ NF-κB）and D-dimer（ D-D） in heart failure model mice. Method： Totally 100 SPF male mice were divided into the normal group（ 20 mice） and the model group（ 80 mice） by random. The heart failure mice model was induced through the intraperitoneal injection with isoproterenol. After the successful modeling,they were randomly divided into the RA group（ Astragali Mongolici Radix,3 g ·kg- 1）,the APS group（ Astragali Mongolici Radix polysaccharideits,3 g·kg- 1）,the positive medicine group（ Digoxin,0. 05 mg·kg- 1） and the model group,with20 mice in each group. The RA group was orally given 3 m L·kg- 1·d- 1Astragali Mongolici Radix extract liquid（ 1g·m L- 1）; the APS group was intraperitoneally injected with 3 m L·kg- 1·d- 1of Astragali Mongolici Radix polysaccharide; the positive drug group was orally given 0. 05 m L·kg- 1·d- 1of digoxin; the model control group was orally given 3 m L·kg- 1·d- 1of sterile saline. All of them were treated for 4 weeks. After the experiment,their apex cordis and myocardial tissues were collected for pathological examination. The blood was drawn from abdominal arteries for detecting the expressions of Hcy,NF-κB and D-D. Result： The expressions of Hcy,NF-κB and D-D in the model group were higher than that of the normal group（ P〈0. 05）. According to the pathological results,the model group showed less myocardial fiber,lighter staining,vague bands and partial replacement of necrotic lesions with scar tissues. Compared with the model group,all of treatment groups showed obvious reductions in Hcy,NF-κB and D-D levels（ P〈0. 05）. The pathological results also showed significant reduction in necrotic lesions in apex cordis and myocardial tissues in all of treatment groups. Compared with the RA group and the Digoxin group,the APS showed more signifi

关 键 词：黄芪 黄芪多糖 同型半胱氨酸 核因子-ΚB D-二聚体 

分 类 号：R285.5[医药卫生—中药学]