IPF中医病机“气络失和”与TGF-β_1/Smads信号传导通路活化的相关性探讨  

Correlation Between Traditional Chinese Medical Pathogenesis ‘Qi-Meridian Disharmony'and TGF-β_1/Smads Signaling Pathway Activation in IPF

作　　者：赵仲雪[1] 庞立健[2] 滑振 刘创[2] 郑炜东 臧凝子[1] 袁佺[1] 杨丽[2] 任延毅 吕晓东[1] 

机构地区：[1]辽宁中医药大学,沈阳110032 [2]辽宁中医药大学附属医院,沈阳110032

出　　处：《中国实验方剂学杂志》2015年第17期204-207,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(81373579);国家自然科学基金青年基金项目(81403290);辽宁省科技厅计划项目(2012225018-202);辽宁省中医临床重点学(专)科服务能力建设项目(2013-LNZYXZK-2);沈阳市科技局计划项目(F14-231-1-13);辽宁省高等学校科学研究一般项目基础研究(L2014369)

摘　　要：目的:探析在特发性肺纤维化(IPF)病程中气络失和与转化生长因子-β1(TGF-β1)/Smads信号转导通路活化的相关性。方法:通过查阅文献,明确气络概念,结合导师吕晓东教授所提出的特发性肺纤维化"肺虚络瘀"病机观,提出气络失和为特发性肺纤维化的中医病机之一。基于吴以岭教授"气络-NEI"理论中气络与神经-内分泌-免疫网络存在高度相关性和内在一致性,神经递质、激素、细胞因子等信息分子及其受体是气络在分子水平上的生物学基础,以气络与细胞信号转导通路的结构、功能、疾病发生机制等为切入点,展开对气络失和与TGF-β1/Smads信号转导通路活化两者相关性的探讨。结果:气络通达内外,遍及周身上下,逐层细分,呈树枝状、网络拓扑状结构与细胞信号通路网络结构相近。气络运行气血津液,传递机体信息,并可双向调节,与信号转导通路传递信息,正负反馈调节功能相似。气络失和所致的痰、虚、瘀与TGF-β1/Smads信号转导通路活化所引起的免疫炎症机制、细胞外基质沉积等相似,且邪经气络逐层深入,与TGF-β1/Smads信号转导通路逐步活化过程相似。益气通络的中药复方治疗特发性肺纤维化的作用机制与抑制TGF-β1/Smads信号转导通路有关。结论:IPF中气络失和与TGF-β1/Smads信号转导通路活化的具有相关性,推测益气通络法可能通过调节TGF-β1/Smads信号转导通路发挥抗纤维化作用。Objective： To probe into the correlation of Qi-meridian disharmony and transforming growth factor-β1（ TGF-β1） /Smads signaling pathway activation in idiopathic pulmonary fibrosis（ IPF）. Method：Literatures were searched to define the Qi-meridian concept. On the basis of the pathogenetic concept of ‘lung deficiency and meridian stasis ＇ for idiopathic pulmonary fibrosis came up by Professor LYU Xiao-dong,we proposed that Qi-meridian disharmony is one of the pathogenesis of IPF. Based on the Qi-meridian-NEI theory proposed by Professor WU Yi-ling,there is a high correlation and internal consistency among Qi-meridian and nerve-endocrine-immune network,and neurotransmitters,hormones,cytokines,other information molecules and their receptors are the biological basis of Qi-meridian at the molecular level. The correlation between Qi-meridian disharmony and TGF-β1/ Smads signal transduction pathway activation was studied based on the structure,function and pathogenesis of Qi-meridian and cell signal transduction pathway. Result： Qi-meridian is distributed throughout the body in a topological structure,which is similar with the cell signal transduction network structure.Qi-meridian carries Qi,blood and body fluid,transfers organism information through signal transduction pathways in a two-way manner,with similar functions in positive or negative feedback and regulation. Sputum,deficiency,blood stasis caused by Qi-meridian disharmony are similar to the inflammatory and extracellular matrix caused by the TGF-β1/ Smads signaling pathway activation. And the pathogenic Qi enters through Qi-meridian layer by layer,which is similar to the gradual activation of TGF-β1/ Smads signaling pathway. The action mechanism of Yiqi Tongluo traditional Chinese medicine compound in the treatment of idiopathic pulmonary fibrosis is correlated with the inhibition of TGF-β1/ Smads signal transduction pathway. Conclusion： Qi-meridian disharmony is correlated with the activation of TGF-β1/ Smads signaling pathway in IPF,s

关 键 词：特发性肺纤维化 气络 转化生长因子-β1/Smads通路 相关性 

分 类 号：R287.6[医药卫生—中药学]