甜瓜种质资源多样性分析与指纹图谱的构建  

Analysis of Germplasm Resources Diversity and Construction of Fingerprinting in Melon(Cucumis melo L.)

作　　者：顾冉[1] 周金蒙[1] 马鸿艳[1] 栾非时[1] 

机构地区：[1]东北农业大学园艺学院,农业部东北地区园艺作物生物学与种质创制重点实验室,黑龙江哈尔滨150030

出　　处：《中国蔬菜》2015年第9期31-38,共8页China Vegetables

基　　金：国家西甜瓜产业技术体系-分子育种岗位项目(CARS-26-02);黑龙江省攻关项目(GC12B112)

摘　　要：采用15个形态学标记和32个CAPS（Cleaved Amplified Polymorphism Sequences，CAPS）标记鉴别73份甜瓜材料。通过15个形态学标记，在遗传相似系数为0．82时将73份甜瓜材料分为3种类型。在分子标记中，被筛选的32对CAPS引物多态性比率为29．63％，PIC值分布在0．22—0．38，平均值为0．34；经过CAPS标记聚类分析，73份甜瓜材料间的遗传相似系数在0．50～0．97之间，当相似系数在0．60时，可以将供试材料分为3种类型。通过构建指纹图谱代码与QR编码，为73份甜瓜材料建立了独特的核酸指纹图谱，每份材料可以相互区别，以便快速对种质资源进行鉴定。The 73 melon （ Cucumis melo L. ）accessions were identified by 15 morphological markers and 32 CAPS markers in this experiment. Using 15 morphological markers, these melons were classified into 3 categories, when the genetic similarity coefficient was 0.82. The polymorphism rate of CAPS markers were 29.63% in molecular markers. The average polymorphism information content （ PIC ） was 0.34 with a range of 0.22-0.38. The result of CAPS cluster analysis showed that the genetic similarity coefficients of these 73 accessions were ranged from 0.50- 0.97. Also, the test materials could be divided into 3 categories, when the genetic similarity coefficient was 0.60. The fingerprinting codes and QR codes had established unique fingerprint for the 73 melon accessions. Each accession could be distinguished from the others. Thus, the germplasm resources could be quickly identified.

关 键 词：甜瓜 形态学标记 CAPS标记 多样性分析 指纹代码 

分 类 号：S652[农业科学—果树学]