微型结球白菜小孢子的培养及植株再生  

Microspore Culture and Plantlet Regeneration in Miniature Heading Chinese Cabbage

作　　者：卢松[1,2] 陶莲[1,2] 吴康云[1,2] 邓英[1,2] 王天文[1,2] 赵大芹[1,2] 

机构地区：[1]贵州省园艺研究所,贵州贵阳550006 [2]贵州省农业科学院园艺工程技术研究中心,贵州贵阳550006

出　　处：《贵州农业科学》2015年第8期30-33,共4页Guizhou Agricultural Sciences

基　　金：贵州省年度攻关项目"芸薹属蔬菜小孢子培养高频胚诱导技术研究及应用"[黔科合NY字(2011)3036];贵州省科技计划项目"贵州辣椒;白菜;萝卜等8种蔬菜种质创新;新品种选育及配套技术研究"[黔科合重大专项(2013)6007];贵州省基金项目"人工培养条件下大白菜游离小孢子胚诱导研究"[黔科合J字(2011)2145];贵州省蔬菜产业技术体系建设项目"贵州省现代农业产业技术体系建设"(GZCYX2014);贵州省年度攻关项目"微型结球白菜优质新品种选育研究"[黔科合NY字(2009)3032];贵州省农科院创新专项"白菜;青菜种质资源创新及新品种选育与示范"[黔农科院自主创新科研专项字(2014)016];贵州省农科院专项"大白菜DH群体的鉴选及新组合选育"[黔农科院院专项(2013)011]

摘　　要：为创新微型结球白菜育种材料,加快微型结球白菜新品种的选育,对微型结球白菜3个不同基因型进行小孢子培养,并对获得的再生植株进行倍性鉴定。结果表明,3个基因型中高山娃娃菜获得胚状体及再生植株,胚诱导成功率为33.3%;高山娃娃菜产胚率为1.4胚/蕾,自然加倍率达85%。经田间鉴定获得2个DH系,人工授粉保存。结论:小孢子诱导成胚过程受基因型影响,小孢子培养技术在微型结球白菜育种上具有一定的应用前景。The microspores of three different genotypes of miniature heading Chinese cabbage varieties were cultured and the ploidy of regenerated plantlets was identified to innovate breeding materials of miniature heading Chinese cabbage and speed up breeding of new miniature heading Chinese cabbage varieties. The results showed that the embryoids and regenerated plantlets can be obtained from Gaoshanwawacai variety only and the embryo induction rate, embryogenic rate and naturally doubling rate is 33,3o//oo, 1.4 embryos/bud and 85O/oo respectively. Two DH lines are identified in field and preserved by artificial pollination. In conclusion, the induction embryogenic process of microspores from miniature heading Chinese cabbage varieties is affected by their genotypes and microspore culture technology is of a certain application prospect in breeding of miniature heading Chinese cabbage.

关 键 词：微型结球白菜 小孢子培养 植株再生 倍性鉴定 

分 类 号：S634.3[农业科学—蔬菜学]