人参保健大米中7种人参皂苷的高效液相色谱测定  

Detection of Seven Ginsenoside Components in Ginseng Health Rice by HPLC

作　　者：韩凤波 

机构地区：[1]吉林农业科技学院中药学院,吉林吉林132101

出　　处：《贵州农业科学》2015年第8期246-248,253,共4页Guizhou Agricultural Sciences

基　　金：吉林省教育厅科研项目"中药保健大米开发研究"[吉教科合字(2012)第309号]

摘　　要：为有效控制人参保健大米的质量,以7种人参皂苷（Rg1,Re,Rb1,Rc,Rb2,Rd,Rf）为指标,研究人参皂苷成分的检测方法。结果表明：在色谱柱为ACQUITY UPLC BEH C18色谱柱（2.1×50mm,1.7μm）,流动相乙腈-水梯度洗脱（0～5min,15%～19%乙腈;5～7min,19%～20%乙腈;7～9min,20%～25%乙腈;9～13min,25%～38%乙腈;13～13.5min,38%～60%乙腈;13.5～14min,48%～15%乙腈）,柱温35℃,流速0.4mL/min,检测波长203nm,进样量2μL的条件下进行检测,7种人参皂苷的线性范围分别为Rg1：5.62～81.0μg/mL,Re：5.18～259.0μg/mL,Rb：14.80～240.0μg/mL,Rc：4.70～235.0μg/mL,Rb：26.26～313.0μg/mL,Rd：5.83～291.5μg/mL,Rf：2.09～104.5μg/mL,其相关系数为0.999 5～0.999 9,线性关系良好,加样回收率为98.8%～100.2%。样品中7种人参皂苷（Rg1,Re,Rf,Rb1,Rc,Rb2,Rd）的含量分别为0.017 2mg/g、0.019 6 mg/g、0.028 8 mg/g、0.042 6 mg/g、0.023 4 mg/g、0.016 4 mg/g和0.035 6mg/g。该方法简便,分离效果好,精确度高,可用于该产品的质量控制。Seven ginsenoside components （Rgi, Re, Rf, Rbl, Rc, Rb2 and Rd） in ginseng health rice were detected by HPLC under the detection conditions of ACQUITY UPLC BEH C18 chromatographic column（2.1×50mm,1.7μm）, mobile phase acetonitrile-water gradient elution （15～19% acetonitrile for 0～5 min, 19～20% acetonitrile for 5～7 min,20～25% acetonitrile for 7～9min,25%～38% acetonitrile for 9～13 min, 38～60% acetonitrile for 13. ～13. 5 min and 48%～15% acetonitrile for 13.5～14 min）, 35℃, 0.4 mL/min flow velocity, 203 nm wavelength and 2 μL sample size to effectively control quality of ginseng health rice. Results：The linear range of Rgl, Re, R{, Rbl, Rc, Rb2and Rd is 5.62～81.0 t～g/mL,5.18--259.0 /～g/mL 14.80～240.0 gg/mL,4.70～235.0 gg/mL,26.26～313.0 txg/ mL,5. 83- 291. 5 vg/mL and 2. 09 ～ 104. 5 t～g/mL respectively. The correlation coefficient reaches 0. 9995～0. 9999 and the recovery is up to 98.8%--100.2%. Rgl, Re, Rf, Rbl, Rc, Rb2and Rd content in the sample is 0. 017 2 mg/g, 0. 019 6 mg/g, 0. 028 8 mg/g, 0. 042 6mg/g, 0. 023 4 mg/g, 0. 016 4 mg/g and 0. 035 6 mg/g respectively. The detection method with simplicity, good separating effect and high accuracy should be used in quality control of ginseng health rice.

关 键 词：人参保健大米 人参皂苷 高效液相色谱法 

分 类 号：S567[农业科学—中草药栽培] R932[农业科学—作物学]