Roux-en-Y胃旁路术对正常SD大鼠肠道菌群的影响  

Effects of Roux-en-Y Gastric Bypass on Intestinal Microbiota of Normal Sprague-Dawley Rats

作　　者：李肖珂 裘年存 杜植鹏[1] 张勤[1] 饶文胜[1] 单成祥[1] 许辉[2] 

机构地区：[1]第二军医大学附属长征医院普外科,上海黄浦200003 [2]中国人民解放军第422医院肿瘤科,广东湛江524005

出　　处：《中国普外基础与临床杂志》2015年第9期1042-1046,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金(项目编号:81300723)~~

摘　　要：目的探讨Roux-en-Y胃旁路手术(Roux-en-Y gastric bypass,RYGB)对正常Sprague-Dawley(SD)大鼠胆胰支、Roux支和共同支肠道菌群的影响。方法将16只SD大鼠按体质量配对后随机分为假手术组(Sham组)和RYGB组,每组8只。RYGB组大鼠行RYGB,Sham组大鼠离断胃空肠后行原位胃和空肠的端端吻合。比较2组大鼠胆胰支、Roux支及共同支上总细菌、双歧杆菌、拟杆菌和乳酸杆菌m RNA的表达水平。结果与Sham组相比较,术后8周RYGB组大鼠的体质量下降,差异有统计学意义(P<0.01)。实时荧光定量PCR结果显示:在总细菌、双歧杆菌及拟杆菌方面,RYGB组大鼠Roux支和共同支上3类细菌的m RNA表达水平均高于Sham组大鼠对应部位(P<0.05),但2组胆胰支上3类细菌的m RNA表达水平比较差异均无统计学意义(P>0.05);RYGB组大鼠Roux支上乳酸杆菌m RNA的表达水平高于Sham组(P<0.05),但2组胆胰支和共同支上乳酸杆菌m RNA的表达水平比较差异均无统计学意义(P>0.05)。结论 RYGB能显著提高正常SD大鼠Roux支和共同支上总细菌、双歧杆菌及拟杆菌m RNA的表达水平,能提高Roux支上乳酸杆菌m RNA的表达水平,但其对胆胰支上上述各类细菌m RNA的表达水平无影响。Objective intestinal microbiota among Dawley （SD） rats. Methods To investigate the effect of Roux-en-Y gastric bypass （RYGB） on the composition of the biliopancreatic limb, the Roux limb, and the common channel in normal Sprague- Sixteen SD rats were randomly divided into sham surgery group （Sham group） and RYGB group, each group enrolled 8 rats. Rats in Sham group underwent sham surgery of end to end anastomosis in situ after cutting off the stomach and jejunum, and rats in RYGB group underwent RYGB. Then quantitative real-time PCR （RT- PCR） method was used to detect the expression of total bacteria, Bifidobacteriurn, Bacteroides, and Lactobacillus mRNA at biliopancreatic limb, the Roux limb, and the common channel. At last the comparison of mRNA in 4 kinds of bacteria was performed. Results Compared with Sham group, the weight of rats in RYGB group was lower at 8 weeks after surgery （P〈0.01）. RT-PCR results showed that, expression levels of total bacteria, Bifidobacterium, and Bacteroides mRNA at the Roux limb and the common channel in RYGB group were higher than corresponding site of rats in Sham group （P〈0.05）, but there was no significant difference at biliopancreatic limb between the 2 groups （P〉0.05）. Expression level of Lactobacillus mRNA at the Roux limb in RYGB group was higher than corresponding site of rats in Sham group （P〈0.05）, but there was no significant difference at biliopancreatic limb and the common channel between the 2 groups （P〉0.05）.Conclusion RYGB can significantly improve expression levels of the total bacteria, Bifidobacterium, and Bacteroides mRNA at Roux limb and the common channel, increase the level of Lactobacillus mRNA at,Roux limb, while has no influence on biliopancreatic limb.

关 键 词：ROUX-EN-Y胃旁路术 肠道菌群 SPRAGUE-DAWLEY大鼠 肥胖 

分 类 号：R656.61[医药卫生—外科学]