猪繁殖与呼吸综合征病毒ZB株的分离与鉴定  

Isolation and identification of the DB strain from Porcine reproductive and respiratory syndrome virus

作　　者：孟日增[1] 刘金华[1] 孟庆峰[1] 石建平[1] 王伟利[1] 吴连鹏[1] 

机构地区：[1]吉林出入境检验检疫局检验检疫技术中心,长春130062

出　　处：《黑龙江畜牧兽医》2015年第9期171-173,176,295,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家质检公益性项目(201010063)

摘　　要：为了确定从高度疑似猪繁殖与呼吸综合征(PRRS)的猪内脏中分离得到的猪繁殖与呼吸综合征病毒(PRRSV)毒株的类型,试验进行了形态学观察、血清学分析、免疫学分析、RT-PCR扩增等一系列鉴定分析。结果表明:从疑似病料中分离获得的病毒可以使Marc-145细胞产生明显病变,而在Vero和PK-15细胞上不出现细胞病变,从而确定试验获得的病毒为PRRSV,命名为ZB株。To determine a type of Porcine reproductive and respiratory syndrome virus （ PRRSV ） strain isolated from the offal of the pig with highly suspected porcine reproductive and respiratory syndrome （PRRS）, a series of identification and analysis were carried out, including morphological observation, serological analysis ; immunalogical analysis, RT - PCR amplification and so on. The results showed that the obtained virus isolated from suspected pathological materials could make Marc - 145 cells to generate obvious lesions, but no lesions were found in Veto and PK - 15 ceils. Thus, the obtained virus was determined as PRRSV and named as ZB strain.

关 键 词：猪繁殖与呼吸综合征病毒(PRRSV) 形态学观察 血清学分析 免疫学分析 RT-PCR扩增 

分 类 号：S852.659.6[农业科学—基础兽医学]